BioModelsapplication/xmlhttps://www.ebi.ac.uk/biomodels/model/download/MODEL1101100000?filename=MODEL1101100000.pdfhttps://www.ebi.ac.uk/biomodels/model/download/MODEL1101100000?filename=MODEL1101100000-biopax3.owlhttps://www.ebi.ac.uk/biomodels/model/download/MODEL1101100000?filename=MODEL1101100000-biopax2.owlhttps://www.ebi.ac.uk/biomodels/model/download/MODEL1101100000?filename=MODEL1101100000_urn.xmlhttps://www.ebi.ac.uk/biomodels/model/download/MODEL1101100000?filename=MODEL1101100000_url.xmlhttps://www.ebi.ac.uk/biomodels/model/download/MODEL1101100000?filename=MODEL1101100000.mhttps://www.ebi.ac.uk/biomodels/model/download/MODEL1101100000?filename=MODEL1101100000.xpphttps://www.ebi.ac.uk/biomodels/model/download/MODEL1101100000?filename=MODEL1101100000.scihttps://www.ebi.ac.uk/biomodels/model/download/MODEL1101100000?filename=MODEL1101100000.pnghttps://www.ebi.ac.uk/biomodels/model/download/MODEL1101100000?filename=MODEL1101100000.vcmlprimaryOK200Arathi G.RNon-curatedordinary differential equation modelL2V4https://www.ebi.ac.uk/biomodels/MODEL11011000009013556falseBioModelsSBMLModelsBakker1997 Glycolysis1997MODEL1101100000Barbara M Bakker, Paul A M Michels, Fred R Opperdoes, H V WesterhoffBarbara M Bakker9013556, In trypanosomes the first part of glycolysis takes place in specialized microbodies, the glycosomes. Most glycolytic enzymes of Trypanosoma brucei have been purified and characterized kinetically. In this paper a mathematical model of glycolysis in the bloodstream form of this organism is developed on the basis of all available kinetic data. The fluxes and the cytosolic metabolite concentrations as predicted by the model were in accordance with available data as measured in non-growing trypanosomes, both under aerobic and under anaerobic conditions. The model also reproduced the inhibition of anaerobic glycolysis by glycerol, although the amount of glycerol needed to inhibit glycolysis completely was lower than experimentally determined. At low extracellular glucose concentrations the intracellular glucose concentration remained very low, and only at 5 mM of extracellular glucose, free glucose started to accumulate intracellularly, in close agreement with experimental observations. This biphasic relation could be related to the large difference between the affinities of the glucose transporter and hexokinase for intracellular glucose. The calculated intraglycosomal metabolite concentrations demonstrated that enzymes that have been shown to be near-equilibrium in the cytosol must work far from equilibrium in the glycosome in order to maintain the high glycolytic flux in the latter.. 6, 272. Microbial Physiology, BioCentrum Amsterdam, Vrije Universiteit, De Boelelaan 1087, NL-1081 HV Amsterdam, BioCentrum Amsterdam, University of Amsterdam, Plantage Muidergracht 12, NL-1018 TV Amsterdam, The Netherlands.arathignair@gmail.comMESCASIn trypanosomes the first part of glycolysis takes place in specialized microbodies, the glycosomes. Most glycolytic enzymes of Trypanosoma brucei have been purified and characterized kinetically. In this paper a mathematical model of glycolysis in the bloodstream form of this organism is developed on the basis of all available kinetic data. The fluxes and the cytosolic metabolite concentrations as predicted by the model were in accordance with available data as measured in non-growing trypanosomes, both under aerobic and under anaerobic conditions. The model also reproduced the inhibition of anaerobic glycolysis by glycerol, although the amount of glycerol needed to inhibit glycolysis completely was lower than experimentally determined. At low extracellular glucose concentrations the intracellular glucose concentration remained very low, and only at 5 mM of extracellular glucose, free glucose started to accumulate intracellularly, in close agreement with experimental observations. This biphasic relation could be related to the large difference between the affinities of the glucose transporter and hexokinase for intracellular glucose. The calculated intraglycosomal metabolite concentrations demonstrated that enzymes that have been shown to be near-equilibrium in the cytosol must work far from equilibrium in the glycosome in order to maintain the high glycolytic flux in the latter.Glycolysis in bloodstream form Trypanosoma brucei can be understood in terms of the kinetics of the glycolytic enzymes.Bakker B M BM, Michels P A PA, Opperdoes F R FR, Westerhoff H V HVextent, AW488255, Sectors, Public Sectors, Tb11, YB, NetrinA, AUTSX5, number, D430049E23Rik, Copyrights, NOVH, CCN3, QM, FBXW4, netrin, Yb, Hek6, Cek6, Public Enterprise, Enterprises, CG2706, fs(1)M104, ENSMUSG00000074119, ERP, APUDoma, Erp, Elkh, Ebi, EBI, Public Domains, Tyrosine-protein kinase receptor EPH-2, EK6, SAP-2, Sap-2, IGFBP9, Public Enterprises, DmelCG4063, IBP-9, neuroendocrine tumour, Kiaa4053, 2.7.10.1, Solute carrier family 6 member 2, L10, Etrp, CT27014, NET1, SLC6A5, Tbl1, TBL1, NAT1, netA, NOVh, Enterprise, NET, Net, Elk, ELK, C130099E04Rik, completeness, Neuronally-expressed EPH-related tyrosine kinase, DmelCG2706, EPH tyrosine kinase 2, DXS648, SAP2, SMAP55, 9330129L11, neuroendocrine tumor, net, neuroendocrine neoplasm, presence., count in organism, Norepinephrine transporter, IGFBP-9, Public, Public Domain, Domains, EPH-like kinase 6, NOV, PlexA1, Domain, Data Base, Plxn1, CG4063, nov, hEK6, CG18657, E-2f, mKIAA4053, E-2g, fs(1)Y[b], l(2)k16213, DmelCG18657, Sector, EPHT2, C130088N23Rik, EG:95B7.8, 2600013D04Rik, PLXN1, DXS648E, netrin AEmbden-Meyerhof-Parnas Pathway, Pathway, glycolysis, Embden Meyerhof Pathway, modified Embden-Meyerhof pathway, anaerobic glycolysis, Embden-Meyerhof pathway., Embden-Meyerhof, Pathways, Embden-Meyerhof Pathway, Embden-Meyerhof-Parnas, Embden-Meyerhof-Parnas pathway, Embden-Meyerhof Pathways, Embden Meyerhof Parnas PathwayTrypanosoma brucei subgroup, nucleocytoplasm, acetylglucosaminyltransferase-like protein, Theoretical Model, Glukose, Characterization, Mbp1, Follow, Embden-Meyerhof, Monohydrate, (DL)-Isomer, Embden Meyerhof Parnas Pathway, Mathematical Models, Relative, Trypanosoma, Cytosolic, primary metabolites, Permutation, hk1-A, Completely, Dextrose, 1, 2, Response Inhibition, Related, Embden-Meyerhof Pathway, CALCULATION, Inhibitory, Very Low Risk, animal, myd, Close, Definite, 3-Propanetriol, Oelsuess, me75, Theoretical Study, Nearby, enzymes, like-acetylglucosaminyltransferase, Determination, Completely Able, COMPLETED, Association, Pathways, Mbp-1, Complete Agreement, Based, D17Mit170, free, T1, DL-glucose, glucose, Biphasic, Had, Near, In Accordance, Agreement, Delta, Relationship, Glycosome, Has, Place, Glyceritol, Lower, Sequence of Planned Assessment Schedule, Adhere To, Complies, Pathway, Papers, Determined, Trypanosoma brucei bruceus, gyltl1b-b, Difference, anaerobic glycolysis, Placement, Measured Tumor Identification, Embden-Meyerhof-Parnas, Tl3, Tl2, predicted, Embden-Meyerhof-Parnas Pathway, organism, Experimental, MEASURED, Non, MDDGA6, mKIAA0609, Algorithm, Theoretical Studies, glycerol, Not, LOWER, Adverse Event Definitely Related to Intervention, Conflict, Adverse Event Related to Intervention, internal to cell, Agree Completely, (alpha-D)-Isomer, KIAA0609, acetylglucosaminyltransferase-like 1A, Hexokinase A, fg, D-Glucose, Hexokinase D, Maintain, Gro, gyltl1b, whole organism, glycerine, Definite Attribution, Trypanosoma bruceus, Very Low, mdc1d, Relation, ATP:D-hexose 6-phosphotransferase, In Accordance With, Specialized, LARGE_HUMAN, Trypanosoma brucei, Embden-Meyerhof pathway, Theoretical Models, experimental procedures, Condition, Characterized, Lowering, MDC1D, glycolysis, enr, D Glucose, brucei brucei, metabolites, Put, Koerper, Hexokinase, Biocatalyst, IPSS-R Risk Category Very Low, Cytosols, Arrangement number, Glucose Monohydrate, Embden-Meyerhof Pathways, Mathematical Model, Per, Placed, gluco-hexose, Full Agreement, Non-, multi-cellular organism, Ordered, experimental, Experimental Models, Glucose, Biocatalysts, Cytosol, Different, secondary metabolites, Trypanosoma (Trypanozoon) brucei, Determine, Available, Calculation, Inhibition, LARGE1, Theoretical, froggy, Gyltl1a, algorithm, Maintenance., Glycerine, glycyl alcohol, Propanetriol, Completely Agree, Relations, Calculated, modified Embden-Meyerhof pathway, Trihydroxypropane, Studies, Experimental Model, Low, Models, protoplasm, Inhibiting, Negation, Complete, methods, Measured, protoplast, experimental section, MDDGB6, Glycerin, ORDER, RELATIVE, LARGE, Theoretic, Near to, Study, BPFD#36, Purification, Enzyme, glycerolum, Mathematical, bruceus, Completion, Algorithms, species, Model, Anhydrous, Possess, Hexokinase II, Embden Meyerhof Pathway, cou, (beta-D)-Isomer, Microbody, body, Glyzerin, Conditions, Model (Theoretical), whole body, Certain, enzyme activity, Related Attribution, Availability, Complies With, Order, Lr, Purified, Calculate, Have, Medical Order, brucei, Fully Agree, Lowered, Basic, 3-Trihydroxypropane, Definitely Related to Intervention, Purify, Basis, COMPLETE, metabolite, IPSS-R Very Low Risk, Glycosomes, Negated, Embden-Meyerhof-Parnas pathway, Anhydrous Dextrose, No, extracellular, Models (Theoretical), Differential, like-glycosyltransferase, concentration, Change, Bra, Closed, Completed, Revised International Prognostic Scoring System for Myelodysplastic Syndrome Very Low Risk Category, glycosyltransferase-like protein LARGE1, GlcPathway, Trypanosoma brucei subgroup, Embden Meyerhof Pathway, enzymes, Trypanosoma brucei bruceus, Biocatalysts, Trypanosoma bruceus, anaerobic glycolysis, Biocatalyst., Embden-Meyerhof, Pathways, Trypanosoma (Trypanozoon) brucei, enzyme activity, Embden-Meyerhof-Parnas, Embden-Meyerhof-Parnas pathway, Embden Meyerhof Parnas Pathway, Trypanosoma brucei, Embden-Meyerhof pathway, Embden-Meyerhof-Parnas Pathway, Trypanosoma, Enzyme, glycolysis, brucei brucei, modified Embden-Meyerhof pathway, bruceus, Embden-Meyerhof Pathway, brucei, Embden-Meyerhof PathwaysfalseBakker1997_Glycolysis This model originates from BioModels Database: A Database of Annotated Published Models (http://www.ebi.ac.uk/biomodels/). It is copyright (c) 2005-2011 The BioModels.net Team. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not.. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92. 2011-06-232005-01-012011-01-10MODEL11011000009013556MODEL11011000005691