BioModelsapplication/xmlhttps://www.ebi.ac.uk/biomodels/model/download/MODEL1504080006?filename=MODEL1504080006.pdfhttps://www.ebi.ac.uk/biomodels/model/download/MODEL1504080006?filename=MODEL1504080006_urn.xmlhttps://www.ebi.ac.uk/biomodels/model/download/MODEL1504080006?filename=MODEL1504080006_url.xmlhttps://www.ebi.ac.uk/biomodels/model/download/MODEL1504080006?filename=MODEL1504080006.vcmlhttps://www.ebi.ac.uk/biomodels/model/download/MODEL1504080006?filename=MODEL1504080006.scihttps://www.ebi.ac.uk/biomodels/model/download/MODEL1504080006?filename=MODEL1504080006.pngprimaryOK200R CostaNon-curatedordinary differential equation modelL2V4https://www.ebi.ac.uk/biomodels/MODEL1504080006falseBioModelsSBMLModelsCosta2015 Central metabolism of E. coli, extended regulated linlog model ( with additional allosteric regulations than the Model1)MODEL1504080006Non KineticRafael S. Costa, Susana VingaRafael S. Costa10.1016/j.bej.2016.01.013, Kinetic modeling is a key aspect of systems biology with biotechnological applications. However, a limitation of building kinetic models of metabolism (particularly from stoichiometric reconstructions of metabolic networks) is that they often ignore the allosteric regulators. This can cause discrepancies in the model predictions. In this paper, we derived an approximated lin-log ODE model of the Escherichia coli central carbon metabolism, with and without metabolite-enzyme regulators. Next, we analyzed the influence of incorporating this level of metabolite-enzyme interactions in the metabolic network by performing several in silico single-gene knockouts and enzyme under-/over-expression changes. Through comparing these model predictions with those generated with a reference mechanistic kinetic model for E. coli, it is shown that including of allosteric regulation affects the flux control patterns over serine production and reveals more details of the model behavior in a general sense. The present work demonstrates that the regulatory (allosteric) structure in metabolic networks plays an essential role to further improve kinetic model prediction capabilities. The incorporation of allosteric regulation interactions in building a kinetic model can lead to different hypotheses in order to suggest enzyme targets for strain design through metabolic engineering.. null, null. IDMEC, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisboa, Portugalrafael.s.costa@tecnico.ulisboa.pt10.1016/j.bej.2016.01.013INESC-ID / IST, University of Lisbonbiochemical pathways, multicellular organism metabolic process, Metabolic Process, biodegradation, Metabolic, degradation, Process, catabolism, Processes, metabolism resulting in cell growth, Metabolic Concepts, Metabolic Concept, metabolic process resulting in cell growth, Metabolic Processes, Anabolism., Concept, Metabolic Phenomena, Metabolism Concepts, single-organism metabolic process, Metabolism, Phenomena, Concepts, biotransformation, secretion, Metabolism Concept, Phenomenon, Metabolism Phenomena, Catabolism, metabolism, Metabolic PhenomenonDesc, DESCR., Description, Descriptive, Descriptor, description, Product Description/AppearancefalseCosta2015 - Central metabolism of E. coli, extended regulated linlog model ( with additional allosteric regulations than the Model1)No description2016-02-222016-02-222015-04-08MODEL1504080006MODEL150408000610.1016/j.bej.2016.01.013