BioModelsapplication/xmlhttps://www.ebi.ac.uk/biomodels/model/download/MODEL2010160002?filename=CCM_ext_Gly.xmlprimary200OKAlexandre OliveiraNon-curatedotherL2V4https://www.ebi.ac.uk/biomodels/MODEL2010160002falseBioModelsSBMLModelsOliveira2020 E. coli Extended Central Carbon Metabolism from Glycerol2021MODEL2010160002Alexandre Oliveira, Joana Rodrigues, Eugénio Campos Ferreira, Lígia Rodrigues, Oscar DiasAlexandre Oliveira10.1371/journal.pcbi.1008704, Acrylic acid is a value-added chemical used in industry to produce diapers, coatings, paints, and adhesives, among many others. Due to its economic importance, there is currently a need for new and sustainable ways to synthesise it. Recently, the focus has been laid in the use of Escherichia coli to express the full bio-based pathway using 3-hydroxypropionate as an intermediary through three distinct pathways (glycerol, malonyl-CoA, and β-alanine). Hence, the goals of this work were to use COPASI software to assess which of the three pathways has a higher potential for industrial-scale production, from either glucose or glycerol, and identify potential targets to improve the biosynthetic pathways yields. When compared to the available literature, the models developed during this work successfully predict the production of 3-hydroxypropionate, using glycerol as carbon source in the glycerol pathway, and using glucose as a carbon source in the malonyl-CoA and β-alanine pathways. Finally, this work allowed to identify four potential over-expression targets (glycerol-3-phosphate dehydrogenase (G3pD), acetyl-CoA carboxylase (AccC), aspartate aminotransferase (AspAT), and aspartate carboxylase (AspC)) that should, theoretically, result in higher AA yields.. 3, 17. Centre of Biological Engineering - University of Minhoid9248@alunos.uminho.pt10.1371/journal.pcbi.1008704University of MinhofalseOliveira2020 - E. coli Extended Central Carbon Metabolism from Glycerol The kinetic model of E. coli central carbon metabolism developed by Millard et al. 2016 was extended to include the production Glycerol, Malonyl-CoA, and Beta-Alanine. This model was then used as a base to insert three independent heterologous pathways for 3-hydroxypropionate and acrylic acid production. 2021-03-092021-03-092020-10-16MODEL2010160002OTHER10.1371/journal.pcbi.1008704