{"database":"BioModels","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Xml":["https://www.ebi.ac.uk/biomodels/model/download/MODEL2506160002?filename=Gosselin2025_Ecoli_bioproduction_sensitive.xml"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"submitter":["Millard"],"curationStatus":["Non-curated"],"modellingApproach":["ordinary differential equation model"],"levelVersion":["L2V4"],"full_dataset_link":["https://www.ebi.ac.uk/biomodels/MODEL2506160002"],"isPrivate":["false"],"repository":["BioModels"],"modelFormat":["SBML"],"omics_type":["Models"],"tokenised_name":["Gosselin2025   Ecoli bioproduction sensitive"],"submissionId":["MODEL2506160002"],"publication_authors":["Thomas Gosselin-Monplaisir, Denis Jallet, Erwana Harscoet, Uttenweiler-Joseph, Stéphanie Heux"],"first_author":["Thomas Gosselin-Monplaisir"],"publication":["10.1101/2025.06.17.659982,\n                            Escherichia coli, a key workhorse in industrial biotechnology, is commonly grown on glucose, which supports rapid growth but leads to acetate overflow. Acetate has long been regarded as a toxic by-product, known to divert carbon away from production pathways, inhibit cellular growth, and reduce productivity. Recent studies suggest that acetate may also have beneficial effects in glucose-grown E. coli, but its potential in bioprocesses remains unexplored. In this study, we systematically investigated acetate’s impact on bioproduction using a kinetic model of glucose and acetate metabolism in E. coli. The model predicts that acetate can enhance bioproduction in glucose-grown E. coli through three mechanisms: (i) by minimizing acetate overflow, thereby reducing carbon loss, (ii) by increasing acetyl-CoA levels, thereby boosting the biosynthetic flux of acetyl-CoA-derived compounds, and (iii) by promoting biomass accumulation, thus improving overall productivity. We experimentally validated the predictions of the model for mevalonate and 3-hydroxypropionate production, where acetate supplementation increased productivity by 117% and 34%, respectively. Our findings provide a valuable framework for optimizing E. coli-based bioprocesses and highlight acetate’s underutilized potential in biotechnology. By leveraging acetate from waste streams as a metabolic booster, this approach could contribute to more sustainable and environmentally friendly bioprocesses.. null, null.\n                            Toulouse Biotechnology Institute"],"submitter_mail":["pierre.millard@insa-toulouse.fr"],"publication_doi":["10.1101/2025.06.17.659982"],"submitter_affiliation":["INRAE"],"additional_accession":[]},"is_claimable":false,"name":"Gosselin2025 - Ecoli_bioproduction_sensitive","description":"Kinetic model for bioproduction via an acetyl-CoA sensitive pathway, as detailed in DOI 10.1101/2025.06.17.659982.","dates":{"last_modification":"2025-06-23","publication":"2025-06-23","submission":"2025-06-16"},"accession":"MODEL2506160002","cross_references":{"biomodels__db":["MODEL2506160002"],"doi":["10.1101/2025.06.17.659982"]}}