BioModelsWilliam ScottNon-curatedother*https://www.ebi.ac.uk/biomodels/MODEL2605020001falseBioModelsOtherModelsScott2026 Dehalobacter syntrophic community metabolic model2026MODEL2605020001William T Scott, Luz Puentes Jácome, Bart Nijsse, Jinsong Wang, Gerben Stouten, Koehorst JJ, Hauke Smidt, Elizabeth Edwards, Peter J Schaap, Robbert KleerebezemWilliam T Scott10.64898/2026.05.05.723060, <h4>ABSTRACT</h4> Organohalide-respiring bacteria (OHRB), such as Dehalobacter , play key roles in the bioremediation of anoxic environments contaminated with chlorinated aromatic compounds. These obligate anaerobes rely on syntrophic interactions to obtain essential resources—hydrogen, acetate, and corrinoid cofactors—from acetogens and fermenters. However, the metabolic interactions enabling complete reductive dehalogenation of compounds like 1,2,4-trichlorobenzene (1,2,4-TCB) to benzene remain incompletely understood. In this study, we asked: (1) What are the key microbial taxa and their functional roles within a Dehalobacter -containing anaerobic microbial community detoxifying chlorinated benzenes? (2) How do syntrophic interactions enable complete dehalogenation of 1,2,4-TCB to benzene under anaerobic conditions? (3) Can genome-resolved metagenomics and genome-scale metabolic modeling elucidate the metabolic dependencies supporting organohalide respiration in complex consortia? To address these questions, we cultivated microbial communities in batch reactors using methanol as electron donor and either 1,2,4-TCB or monochlorobenzene (MCB) as electron acceptor. In active MCB-fed cultures, benzene increased from 0 to 62.3µmol per bottle while MCB decreased from 88.3 to 22.0µmol per bottle over 120 days, with this pattern repeating across multiple substrate additions. Using genome-resolved metagenomics to identify dominant taxa and select 12 high-quality metagenome-assembled genomes (MAGs) for modeling, we reconstructed genome-scale metabolic models (GEMs) to identify candidate metabolic interactions and predict syntrophic dependencies that may support organohalide respiration in these consortia. Community flux sampling predicted that methanol, H 2 , acetate, and CO 2 formed the dominant exchange backbone of the modeled community, while also indicating competition for shared electron donors between the two Dehalobacter populations. Model-guided minimal-community analysis further identified a narrow dechlorinating core in which all feasible minimal consortia retained a Dehalobacter member together with Methanothrix . These results provide a modeling-informed framework for hypothesis generation and future experimental validation of anaerobic consortia relevant to bioremediation.. null, null. Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907william.scott@wur.nl10.64898/2026.05.05.723060Wageningen University & ResearchfalseScott2026 - Dehalobacter syntrophic community metabolic modelGenome-resolved metagenomics and constraint-based metabolic modeling were used to define and analyze a 12-member anaerobic, Dehalobacter-containing mixed community enriched on chlorinated benzenes. The study examines pairwise ecological interactions, community-level cross-feeding, and minimal dechlorinating consortia to identify the metabolic dependencies supporting reductive dehalogenation of 1,2,4-trichlorobenzene to benzene under methanol-fed anoxic conditions.2026-08-302026-09-022026-05-02MODEL260502000110.64898/2026.05.05.723060