BioModelsFreddy Castillo AlfonsoNon-curatedconstraint-based modelL3V1Not Peer Reviewedhttps://www.ebi.ac.uk/biomodels/MODEL2608050001falseBioModelsModelsSBMLCastillo2026 Genome scale metabolic model of Leucoagaricus gongylophorus2026MODEL2608050001Freddy Castillo-AlfonsoFreddy Castillo-Alfonso, Juan Carlos Sigala Alanis, Juan Gabriel Vigeras Ramirez, Roberto Olivares-Hernándeznull, The fungus Leucoagaricus gongylophorus LEU18496 establishes a mutualistic relationship with leaf-cutting ants by expressing enzymes capable of degrading complex plant polymers. These enzymes are attractive for potential applications in biotechnological bioprocess. To explore the metabolism of this organism and its capabilities for enzyme production, the first genome-scale metabolic model was constructed (the iFC550 model). The model comprises 783 reactions, 635 metabolites and 550 GPR. Growth kinetics on glucose data was used to verify the ATP maintenance coefficient and to validate prediction of the low growth rate observed experimentally for this organism. Flux variability analysis (FVA) and gene deletion simulations identified a high degree of robustness in lipid and energy metabolism, as well as substantial flexibility in the pentose phosphate pathway (PPP) and amino acid metabolism. Transcriptomic data was integrated to show that, in the absence of the oxidative phase of the PPP, NADPH supply relies mainly on malic enzyme and isocitrate dehydrogenase, both active in the model and supported by differential expression. Finally, Pareto front analyses revealed that cellulase production requires more energy than laccase production, demonstrating the metabolic cost of enzyme synthesis. This work provides a model to explore fungal metabolism and to design strategies for enzymatic production optimization.. null, null. Posgrado en Ciencias Naturales e Ingeniería, Universidad Autónoma Metropolitana Unidad Cuajimalpa, Ciudad de México 05370, México.fcastillo2@sdsu.eduUniversidad Autonoma MetropolitanafalseCastillo2026 - Genome-scale metabolic model of Leucoagaricus gongylophorusNo description2026-08-052026-09-022026-08-05MODEL2608050001