biostudies-arrayexpressCBDM LabMus musculusMicroArraySuite 5.0https://www.ebi.ac.uk/biostudies/studies/E-GEOD-1112Four independent chip hybridization with RNAs from four independent RTOC cultures.biostudies-arrayexpressHybridization - Title: Affymetrix Generic Hybridization. Description:MIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsFeature Extraction - Title: Affymetrix CEL analysis. Description:MetabolomicsUnknownTranscriptomicsGenomicsProteomicsThymocytes displaying self-reactive T cell receptors usually undergo negative selection in the thymus. Here we demonstrate that agonist peptides can promote positive selection of immature double-positive thymocytes into distinct lineages, varying with the agonist concentration and the animal's age. Microarray gene expression analyses showed broad transcriptional alterations in a set of transcripts associated with the innate immune system, as well as silencing of CD8 beta expression. The resulting CD8 alpha alpha T cells showed a rapid effector cytokine response. Hence, T cells displaying self-reactive receptors can have the gene expression profile and phenotypic characteristics of innate immune cells.transcription profiling by arrayMus musculusSelf-reactivity in thymic double-positive cells commits cells to a CD8 alpha alpha lineage with characteristics of innate immune cells.Tetsuya Yamagata, Diane Mathis, Christophe BenoistCBDM LabfalseTranscription profiling of mouse RTOC cell culturesFour independent chip hybridization with RNAs from four independent RTOC cultures.2007-11-08T00:00:00Z2023-08-01T05:33:47.821Z2022-02-03T05:33:12.188ZE-GEOD-111215133507EFO_000276810.1038/ni1070