biostudies-arrayexpressHubert RehrauerHomo sapiensMicroArraySuite 5.0https://www.ebi.ac.uk/biostudies/studies/E-GEOD-14461This SuperSeries is composed of the following subset Series:; GSE14383: Effects of chronic exposure of human bronchial epithelial cells to low doses of cigarette smoke condensate; GSE14385: Response of bronchial epithelial cells to low doses of cigarette smoke condensate and subsequent demethylation agent Experiment Overall Design: Refer to individual Seriesbiostudies-arrayexpressSample Processing - BEAS-2B cells were treated twice a week with 20µg/ml of CSC (~IC20 in MTT assay, data not shown). Control cell line was treated with the same volume of DMSO. After the addition of CSC or DMSO, cells were maintained in the same medium until the next passage.Growth Protocol - BEAS-2B cells were cultured in Bronchial Epithelial cell Growth Medium (BEGM), prepared by supplementing Bronchial Epithelial Basal Medium with SingleQuotesTM (Lonza, Switzerland) which contains retinoic acid, epidermal growth factor, epinephrine, transferrin, triiodothyronin, insulin, hydrocortisone, antimicrobial agents, and bovine pituitary extract. In addition, BEGM medium was supplemented with 10% FCS, penicillin/streptomycin and 2mM glutamate.Sample Processing - BEAS-2B cells were treated twice a week with 5µg/ml of CSC. Control cell line was treated with the same volume of DMSO.Labeling - Biotinylated cRNA were prepared from 2 ug total RNA using Affymetrix GeneChip One-Cycle Target Labeling and Control Reagents according to the manufacturer’s protocol.Growth Protocol - BEAS-2B cells were cultured in Bronchial Epithelial cell Growth Medium (BEGM), prepared by supplementing Bronchial Epithelial Basal Medium with SingleQuotesTM (Lonza, Switzerland) which contains retinoic acid, epidermal growth factor, epinephrine, transferrin, triiodothyronin, insulin, hydrocortisone, antimicrobial agents, and bovine pituitary extract. In addition, BEGM medium was supplemented with 10% FCS, penicillin/streptomycin and 2mM glutamate.Ten lung cancer cell lines were cultured in RPMI-1640 medium (Gibco, Invitrogen), supplemented with 10 % FCS and penicillin/streptomycin.Nucleic Acid Extraction - Total RNA from cell lines was extracted using the RNeasy Mini kit (Qiagen, Switzerland) according to the manufacturer’s protocol. RNA was isolated from BEAS-2B cells treated chronically for one month with CSC or DMSO as well as untreated cells. The quality of RNA samples was tested using capillary electrophoresis (Agilent Bioanalyzer, Switzerland).MIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsAssay Data Transformation - Raw gene expression data generated by the GeneChip Operating Software (Affymetrix) were normalized for all probe sets on the array using the gcrma method.Feature Extraction - Title: Affymetrix CEL analysis. Description:UnknownTranscriptomicsGenomicsProteomicstranscription profiling by arrayHomo sapiensHubert RehrauerfalseTranscription profiling of human bronchial epithelial cells to low doses of cigarette smoke condensateThis SuperSeries is composed of the following subset Series:; GSE14383: Effects of chronic exposure of human bronchial epithelial cells to low doses of cigarette smoke condensate; GSE14385: Response of bronchial epithelial cells to low doses of cigarette smoke condensate and subsequent demethylation agent Experiment Overall Design: Refer to individual Series2009-08-09T00:00:00Z2023-08-18T10:05:30.472Z2022-03-14T13:14:25.028ZE-GEOD-14461GSE14461EFO_0002768