{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Thomas GRAF"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-17316"],"description":["Transcription factor induced reprogramming of one specialized cell type into another is a promising approach for regenerative medicine. However, the process still remains poorly understood, in large part because of the lack of adequate experimental models. Here we describe a robust cell reprogramming system consisting of a B cell line with an inducible form of C/EBPa that can be converted into macrophages with essentially 100% efficiency in only 2 to 3 days. The conversion involves reciprocal changes in cell surface antigen expression, increase in cell granularity and size, alterations in cellular structures, formation of membrane extensions, acquisition of phagocytic capacity and an increased inflammatory responsiveness as well as migratory activity. Analysis of the transcriptome shows complex reciprocal regulation of B cell and macrophage genes, including transcription factors required for the formation of the two lineages. The fact that the cells become irreversibly committed to a macrophage fate within 1 to 2 days after activation of C/EBPa show that they are truly reprogrammed. The system should be useful to study epigenetic and cell biological mechanisms of transcription factor induced cell reprogramming. time course analysis. 6 duplicated conditions: 12 samples. control: HAFTL cells time points: vehicle control, 3h, 12h, 24h, 48h."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Hybridization - Hybridized according to Affymetrix Hybridization protocol","Nucleic Acid Extraction - Trizol reagent","Labeling - Biotynilated according to Affymetrix labeling protocol"],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"data_protocol":["Assay Data Transformation - ID_REF = <br>VALUE = MAS 5 signal<br>ABS_CALL = present/absent call<br>DETECTION P-VALUE = p-value","Feature Extraction - Processed using the Affymetrix Gene Chip Operating System","Image Adquisition - Scanned according to Affymetrix scanning protocol"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"pubmed_abstract":["Here we describe a lineage reprogramming system consisting of a B cell line with an estradiol-inducible form of C/EBPalpha where cells can be converted into macrophage-like cells at 100% efficiency within 2 to 3 days. The reprogrammed cells are larger, contain altered organelle and cytoskeletal structures, are phagocytic, and exhibit an inflammatory response. Time-lapse experiments showed that the cells acquire a macrophage morphology and increased migratory activity as early as 10 hr. During induction, thousands of genes become up- or downregulated, including several dozen transcription and chromatin-remodeling factors. Time-limited exposure of cells to the inducer showed that the reprogrammed cells become transgene independent within 1 to 2 days. The reprogramming can be inhibited, at least partially, by perturbation experiments with B cell and macrophage transcription factors. The tightness, robustness, and speed of the system described make it a versatile tool to study biochemical and biological aspects of lineage reprogramming."],"study_type":["transcription profiling by array"],"species":["Mus musculus"],"pubmed_title":["A robust and highly efficient immune cell reprogramming system."],"pubmed_authors":["Francesca Rapino","Alexis Schubert","Thien-Phong Vu Manh","Luisa De Andres","Timo Zimmermann","Lars Bussmann","Thomas GRAF","Thomas Graf","Bussmann LH, Schubert A, Vu Manh TP, De Andres L, Desbordes SC, Parra M, Zimmermann T, Rapino F, Rodriguez-Ubreva J, Ballestar E, Graf T","Sabrina Desbordes","Maribell Parra"],"additional_accession":[]},"is_claimable":false,"name":"Reprogramming of a B cell line into macrophages","description":"Transcription factor induced reprogramming of one specialized cell type into another is a promising approach for regenerative medicine. However, the process still remains poorly understood, in large part because of the lack of adequate experimental models. Here we describe a robust cell reprogramming system consisting of a B cell line with an inducible form of C/EBPa that can be converted into macrophages with essentially 100% efficiency in only 2 to 3 days. The conversion involves reciprocal changes in cell surface antigen expression, increase in cell granularity and size, alterations in cellular structures, formation of membrane extensions, acquisition of phagocytic capacity and an increased inflammatory responsiveness as well as migratory activity. Analysis of the transcriptome shows complex reciprocal regulation of B cell and macrophage genes, including transcription factors required for the formation of the two lineages. The fact that the cells become irreversibly committed to a macrophage fate within 1 to 2 days after activation of C/EBPa show that they are truly reprogrammed. The system should be useful to study epigenetic and cell biological mechanisms of transcription factor induced cell reprogramming. time course analysis. 6 duplicated conditions: 12 samples. control: HAFTL cells time points: vehicle control, 3h, 12h, 24h, 48h.","dates":{"release":"2009-11-22T00:00:00Z","modification":"2023-08-08T19:00:50.421Z","creation":"2021-10-06T15:40:38Z"},"accession":"E-GEOD-17316","cross_references":{"GEO":["GSE17316"],"pubmed":["19896445"],"EFO":["EFO_0002768"],"doi":["10.1016/j.stem.2009.10.004"]}}