{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Sridar Chittur"],"study_type":["transcription profiling by array"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-17869"],"description":["These samples are part of the MMRRC study conducted at UAlbany  Keywords: strain comparison Whole brains from C57BL/6 mice were compared to those from estrogen receptor knockout mice on the same background. Six samples (3 male and 3 female) from each strain (wild type B6, ERalphaKO, ERbetaKO) were compared in this study. These were processed on Affymetrix Mouse 430 plus 2.0 arrays to obtain expression data for each cell ine."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - total RNA was extracted from brain tissue using Trizol protocol followed by RNEasy cleanup protocol","Hybridization - Standard Affymetrix protocol for Mouse 430 plus 2.0 arrays","Sample Processing - whole brains were extracted and flash frozen in liquid nitrogen followed by storage at -80oC","Labeling - 1ug of total RNA was labeled according to Standard Affymetrix  Eukaryotic protocol for Mouse  430 plus 2.0 arrays"],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Sridar Chittur","Marcy Kuentzel","Paulette McCormick","Gerald Bothe"],"data_protocol":["Feature Extraction - Raw CEL files were processed using Affymetrix Gene Chip Operating Software using MAS5","Assay Data Transformation - ID_REF = <br>VALUE = MAS 5.0 Signal<br>ABS_CALL = indicating whether the transcript was present (P), absent (A), or marginal (M)<br>DETECTION P-VALUE =","Image Adquisition - Standard Affymetrix protocol for Mouse 430 plus 2.0 arrays"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of Estrogen receptor related gene expression in C57BL/6 vs ERKO-alpha and ERKO-beta mice","description":"These samples are part of the MMRRC study conducted at UAlbany  Keywords: strain comparison Whole brains from C57BL/6 mice were compared to those from estrogen receptor knockout mice on the same background. Six samples (3 male and 3 female) from each strain (wild type B6, ERalphaKO, ERbetaKO) were compared in this study. These were processed on Affymetrix Mouse 430 plus 2.0 arrays to obtain expression data for each cell ine.","dates":{"release":"2009-08-29T00:00:00Z","modification":"2023-08-06T02:31:36.643Z","creation":"2022-03-09T13:14:12.174Z"},"accession":"E-GEOD-17869","cross_references":{"GEO":["GSE17869"],"EFO":["EFO_0002768"]}}