{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Gavin Pickett"],"organism":["Homo sapiens"],"software":["MicroArraySuite 5.0"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-18235"],"description":["Nine cigarette smoke condensates (CSCs) were produced under a standard ISO smoking machine regimen and one was produced by a more intense smoking machine regimen. These CSCs were used to treat primary normal human bronchial epithelial cells for 18 hours. Experiment Overall Design: Primary human bronchial/tracheal epithelial cells were grown in culture and treated with 10 different sources of cigarette smoke condensates."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Processing - NHBE cells were treated for 18 hr with cigarette smoke condensates (CSCs) prepared from 5 commercial and 4 research cigarettes, at doses of ~ 4 μg/ml nicotine.","Growth Protocol - Primary human bronchial/tracheal epithelial (NHBE) cells were grown in tissue culture.","Labeling - Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Affymetrix One-Cycle Target Labeling Kit).","Nucleic Acid Extraction - Qiagen RNeasy used for total RNA extraction and was performed according to manufacture's instructions."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","MAGE-TAB Files","Array Designs"],"data_protocol":["Assay Data Transformation - The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.","Feature Extraction - Title: Affymetrix CEL analysis. Description:"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"study_type":["transcription profiling by array"],"species":["Homo sapiens"],"pubmed_authors":["Gavin Pickett"],"additional_accession":[]},"is_claimable":false,"name":"Transcription profiling of human primary bronchial/tracheal epithelial cells treated with 10 different sources of cigarette smoke condensates","description":"Nine cigarette smoke condensates (CSCs) were produced under a standard ISO smoking machine regimen and one was produced by a more intense smoking machine regimen. These CSCs were used to treat primary normal human bronchial epithelial cells for 18 hours. Experiment Overall Design: Primary human bronchial/tracheal epithelial cells were grown in culture and treated with 10 different sources of cigarette smoke condensates.","dates":{"release":"2009-10-03T00:00:00Z","modification":"2023-08-06T05:40:07.344Z","creation":"2022-03-10T05:25:27.537Z"},"accession":"E-GEOD-18235","cross_references":{"GEO":["GSE18235"],"EFO":["EFO_0002768"]}}