{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Masahiro Uchino"],"organism":["Homo sapiens"],"software":["MicroArraySuite 5.0"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-18903"],"description":["We investigated the differentially expressed genes between MCF-7 and MCF-7-14, and estimated the similarities of expression profiles between MCF-7-14 and MDA-MB-231. We identified genes differentially expressed between non-invasive/non-metastatic and invasive/metastatic breast cancer cells. Experiment Overall Design: We performed microarray analysis as following design: MCF-7 versus MCF-7-14 versus MDA-MB-231 cells."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - RNAeasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.","Labeling - In vitro transcription reactions were performed using a GeneChip IVT Labeling Kit, which includes T7 RNA polymerase and Biotin-labeled ribonucleotides.","Sample Processing - MCF-7,  MCF-7-14  and  MDA-MB-231  cells  were  synchronized  in  S-phase  with  the thymidine-hydroxyurea  block  method.","Growth Protocol - Cell lines were maintained in RPMI 1640 (Sigma) containing 10% fetal bovine serum (FBS) and antibiotic-antimycotic."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","Additional Files","MAGE-TAB Files","Array Designs"],"data_protocol":["Assay Data Transformation - The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.","Feature Extraction - Title: Affymetrix CEL analysis. Description:"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"study_type":["transcription profiling by array"],"species":["Homo sapiens"],"pubmed_authors":["Masahiro Uchino"],"additional_accession":[]},"is_claimable":false,"name":"Transcription profiling of human MCF-7 and MCF-7-14 cells","description":"We investigated the differentially expressed genes between MCF-7 and MCF-7-14, and estimated the similarities of expression profiles between MCF-7-14 and MDA-MB-231. We identified genes differentially expressed between non-invasive/non-metastatic and invasive/metastatic breast cancer cells. Experiment Overall Design: We performed microarray analysis as following design: MCF-7 versus MCF-7-14 versus MDA-MB-231 cells.","dates":{"release":"2009-11-14T00:00:00Z","modification":"2022-11-26T20:51:45.227Z","creation":"2022-02-03T19:31:34.836Z"},"accession":"E-GEOD-18903","cross_references":{"GEO":["GSE18903"],"EFO":["EFO_0002768"]}}