{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Yiqiang Wang"],"study_type":["transcription profiling by array"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-23347"],"description":["In order to find out the vital genes during the corneal neovascularization (CNV), we set up two CNV models, one being induced by suture placement and the other by alkali burn to the corneal center. Young adult Balb/c and C57BL/6 were used. The corneas were checked daily for CNV development and some of the corneas were removed at two pre-determined time points for RNA extraction. Preliminary experiment indicated slightly different dynamics in suture- or chemical burn-induced CNV. Vigorous growth appeared at day 5 and day 6 in S-CNV and CB-CNV, and reached maximum length around day 10 and day 14, respectively, in S-CNV and CB-CNV. To check whether genetic MHC background has any effect on development of CNV, we compared CB-CNV in Balb/c and C57BL/6 mice at day 6. Corneas from Balb/c mice were isolated 5 and 10 days after suture-induced CNV, and 6 and 14 days after chemical burn-induced CNV. Corneas from C57BL/6 mice were isolated 6 days after chemical burn-induced CNV. Each time point had two or three biological replications."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Processing - CNV induction with suture or chemical burn (NaOH).","Labeling - Labeled using CapitalBio cRNA Amplification and Labeling Kit (Cat.No.360060,CapitalBio,Beijing).","Hybridization - Standard CapitalBio methods.","Nucleic Acid Extraction - Total RNA extracted using Trizol reagent (Invitrogen, Gaithersburg, MD, USA), concentrated using ethanol precipitation and purified using NucleoSpin® RNA clean-up Kit (MACHEREY-NAGEL,Germany)."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Changkai Jia","Yiqiang Wang","Chunxia Wang","Lingling Yang","Yiqang Wang","Shengwei Ren"],"data_protocol":["Image Adquisition - Luxscan 10KA (CapitalBio).","Assay Data Transformation - ID_REF = <br>Cy5 Intensity = <br>Cy3 Intensity = <br>VALUE = Normalized log2 (Cy5 intensity/Cy3 intensity) ratio<br>PRE_VALUE = Normalized (Cy5 intensity/Cy3 intensity) ratio","Feature Extraction - Using Luxscan 3.0 Image Analysis Software (CapitalBio), analyzed microarray images, converted image signal to digital signal, then normalized based on LOWESS  program."],"additional_accession":[]},"is_claimable":false,"name":"Gene Expression Profiling In Murine Corneal Neovascularization Models","description":"In order to find out the vital genes during the corneal neovascularization (CNV), we set up two CNV models, one being induced by suture placement and the other by alkali burn to the corneal center. Young adult Balb/c and C57BL/6 were used. The corneas were checked daily for CNV development and some of the corneas were removed at two pre-determined time points for RNA extraction. Preliminary experiment indicated slightly different dynamics in suture- or chemical burn-induced CNV. Vigorous growth appeared at day 5 and day 6 in S-CNV and CB-CNV, and reached maximum length around day 10 and day 14, respectively, in S-CNV and CB-CNV. To check whether genetic MHC background has any effect on development of CNV, we compared CB-CNV in Balb/c and C57BL/6 mice at day 6. Corneas from Balb/c mice were isolated 5 and 10 days after suture-induced CNV, and 6 and 14 days after chemical burn-induced CNV. Corneas from C57BL/6 mice were isolated 6 days after chemical burn-induced CNV. Each time point had two or three biological replications.","dates":{"release":"2011-02-08T00:00:00Z","modification":"2023-08-09T12:26:06.972Z","creation":"2022-03-10T05:39:25.324Z"},"accession":"E-GEOD-23347","cross_references":{"GEO":["GSE23347"],"EFO":["EFO_0002768"]}}