biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsChih Tsaitranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-24338Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs. One-condition experment, gene expression of 3A6biostudies-arrayexpressLabeling - standard Affymetrix proceduresHybridization - standard Affymetrix proceduresSample Processing - No treatmentNucleic Acid Extraction - Total RNA was extracted from samples using QIAGEN RNeasy Mini KitGrowth Protocol - 3A6 MSCs was kept in DMEM-LG medium with 10% FBSMIAME ScoreRaw DataOrganizationAssays and DataProcessed DataAdditional FilesMAGE-TAB FilesArray DesignsShih-Chieh HungChih TsaiChih-Chien TsaiChun-Li ChenAssay Data Transformation - ID_REF = <br>VALUE = MAS5 signal<br>ABS_CALL =Image Adquisition - Scanned on Affymatrix GeneArray® scanner and images was analyzed by GeneChip Operating Software (Affymatrix, USA)Feature Extraction - GeneChip Operating Software (Affymatrix, USA) was used for background subtraction and MAS5 normalization.falseHomo sapiens immortalized MSCs: 3A6Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs. One-condition experment, gene expression of 3A62010-09-25T00:00:00Z2022-11-27T10:00:29.607Z2022-02-03T13:20:38.579ZE-GEOD-24338GSE24338EFO_0002768