biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsstephanie huguettranscription profiling by arrayZea maysZea mayshttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-24624affy_cellwall_maize - affy_cellwall_maize - Maize, Zea mays, is one of the most widely grown crops in the world as it provides cereal grain for human purposes and feedstock for cattle. Maize is increasingly used as a biomass fuel, such as ethanol. Studies have shown that maize stem is made up of different lignified cell types: epidermis, sclerenchyma (a few layers under the epidermis and surrounding vascular bundles in the rind), parenchyma (mostly lignified near the epidermis). One of the aims of this work is to find out if it is the proportion of these different cell types or their number (especially for vascular bundles) which influences the degree of digestibility of maize lines. For that the objective of our work is to develop a genomic strategic approach allowing an improvement of traits involved in cell wall degradability. The work will include the mapping of genes considered as strategic in the cell wall biosynthesis, the search of new genes of interest through bio-analysis, the functional analysis of genes suspected to be of importance in cell wall pathway, the search of candidate underlying QTL, and transcriptomic approaches in order to find genes co-regulation, transcription.-Comparison of internodes from 5 maize lines grown in field conditions 10 arrays - maize; organ comparisonbiostudies-arrayexpressNucleic Acid Extraction - ge2bl2:100ug.Nucleic Acid Extraction - ge5bl1:90ug.Hybridization - labelled extract quantity: 10ugNucleic Acid Extraction - ge4bl1:80ug.Sample Processing - no treatmentNucleic Acid Extraction - ge3bl2:20ug.Nucleic Acid Extraction - ge1bl2:60ug.Nucleic Acid Extraction - ge3bl1:70ug.Nucleic Acid Extraction - ge1bl1:60ug.Labeling - labelling Biotin direct, amplification=yes, cRNA 20 ug.Nucleic Acid Extraction - ge4bl2:20ug.Nucleic Acid Extraction - ge5bl2:20ug.Growth Protocol - stem - Planting date: 21/04/2009 Pool de 5 plantes/ lignée/repetition conditions météo en Juillet pendant les prélèvements (station météo des Verrines) RG: Rayonnement global horaire (Joules/cm2) 202 T: Température instantanée horaraire (degrés Celsuis)20.27 U: humidité instantanée horaire (poucentage) 53Nucleic Acid Extraction - ge2bl1:60ug.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsMagalie Pichonstephanie huguetSandrine BalzergueStephanie HuguetMarie-Laure Martin-MagnietteFeature Extraction - The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).Assay Data Transformation - ID_REF = ID number<br>VALUE = Normalized data by GCRMAImage Adquisition - GCOS, Biotin:pmt voltage 570nm,650V,laser power 1falseUnderstanding the Diversity of Maize Cell Wall (affy_cellwall_maize)affy_cellwall_maize - affy_cellwall_maize - Maize, Zea mays, is one of the most widely grown crops in the world as it provides cereal grain for human purposes and feedstock for cattle. Maize is increasingly used as a biomass fuel, such as ethanol. Studies have shown that maize stem is made up of different lignified cell types: epidermis, sclerenchyma (a few layers under the epidermis and surrounding vascular bundles in the rind), parenchyma (mostly lignified near the epidermis). One of the aims of this work is to find out if it is the proportion of these different cell types or their number (especially for vascular bundles) which influences the degree of digestibility of maize lines. For that the objective of our work is to develop a genomic strategic approach allowing an improvement of traits involved in cell wall degradability. The work will include the mapping of genes considered as strategic in the cell wall biosynthesis, the search of new genes of interest through bio-analysis, the functional analysis of genes suspected to be of importance in cell wall pathway, the search of candidate underlying QTL, and transcriptomic approaches in order to find genes co-regulation, transcription.-Comparison of internodes from 5 maize lines grown in field conditions 10 arrays - maize; organ comparison2010-12-07T00:00:00Z2023-08-10T00:21:22.5Z2022-03-06T23:31:01.187ZE-GEOD-24624GSE24624EFO_0002768