{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Tieliu Shi"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-30222"],"description":["This SuperSeries is composed of the SubSeries listed below. Refer to individual Series.  The sequencing samples of these two RNA-Seq batches 1 and 2 are the same, but they were sequenced twice and in different length. We used these two RNA-Seq batches to carry out two different studies."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Standard Illumina mRNA-Seq protocol and reagents"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Feature Extraction - We aligned all the reads onto human reference genome hg19 (download from UCSC http://genome.ucsc.edu/) using the software Tophat (http://tophat.cbcb.umd.edu/) with default parameters."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"pubmed_abstract":["In their expression process, different genes can generate diverse functional products, including various protein-coding or noncoding RNAs. Here, we investigated the protein-coding capacities and the expression levels of their isoforms for human known genes, the conservation and disease association of long noncoding RNAs (ncRNAs) with two transcriptome sequencing datasets from human brain tissues and 10 mixed cell lines. Comparative analysis revealed that about two-thirds of the genes expressed between brain and cell lines are the same, but less than one-third of their isoforms are identical. Besides those genes specially expressed in brain and cell lines, about 66% of genes expressed in common encoded different isoforms. Moreover, most genes dominantly expressed one isoform and some genes "],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"pubmed_title":["Comparative analysis of human protein-coding and noncoding RNAs between brain and 10 mixed cell lines by RNA-Seq."],"pubmed_authors":["Tieliu Shi","Chen G, Yin K, Shi L, Fang Y, Qi Y, Li P, Luo J, He B, Liu M, Shi T"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of brain and various cell lines","description":"This SuperSeries is composed of the SubSeries listed below. Refer to individual Series.  The sequencing samples of these two RNA-Seq batches 1 and 2 are the same, but they were sequenced twice and in different length. We used these two RNA-Seq batches to carry out two different studies.","dates":{"release":"2011-12-12T00:00:00Z","modification":"2023-08-20T16:44:29.831Z","creation":"2022-03-10T02:45:55.605Z"},"accession":"E-GEOD-30222","cross_references":{"pubmed":["22140575"],"ENA":["SRP007351"],"EFO":["EFO_0003738"],"doi":["10.1371/journal.pone.0028318"]}}