{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Jörg Heierhorst"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-30417"],"description":["In order to identify potential transcriptional targets of ASCIZ, we performed a microarray analysis of three wildltype and ASCIZ(ATMIN/ZNF822)-deficient littermate single-passage MEF cultures.  Only 22 and 25 genes were found to be down- or upregulated in the KO, respectively, by >1.5-fold (p<0.05).  The most severely downregulated gene was Dynll1, which was confirmed by Norhtern and Western blot in mulitple embryonic tissues. Primary MEFs were isolated from embryonic day 12.5 embryos (3 wildtype, 3 knockouts), and passaged once before isolation of total RNA."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Growth Protocol - 48 hours Dulbecco's Modified Eagle's Medium 10% fetal bovine serum in 10-cm dish, then transfer to 15-cm dish in same medium","Hybridization - Standard Illumina hybridization protocol","Nucleic Acid Extraction - Total RNA was extracted using Rneasy Mini kit (Qiagen).","Labeling - Biotinylation using Ambion Total Prep RNA Amplification Kit (Cat. No. IL1791)."],"figure_sub":["MIAME Score","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"data_protocol":["Feature Extraction - All samples were normalised by quantile normalisation method using lumi package in R statistical software.","Assay Data Transformation - ID_REF = <br>VALUE = quantile normalized","Image Adquisition - Standard Illumina scanning protocol"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"pubmed_abstract":["The highly conserved DYNLL1 (LC8) protein was originally discovered as a light chain of the dynein motor complex, but is increasingly emerging as a sequence-specific regulator of protein dimerization with hundreds of targets and wide-ranging cellular functions. Despite its important roles, DYNLL1's own regulation remains poorly understood. Here we identify ASCIZ (ATMIN/ZNF822), an essential Zn(2+) finger protein with dual roles in the DNA base damage response and as a developmental transcription factor, as a conserved regulator of Dynll1 gene expression. DYNLL1 levels are reduced by ∼10-fold in the absence of ASCIZ in human, mouse and chicken cells. ASCIZ binds directly to the Dynll1 promoter and regulates its activity in a Zn(2+) finger-dependent manner. DYNLL1 protein in turn interacts w"],"study_type":["transcription profiling by array"],"species":["Mus musculus"],"pubmed_title":["ATM substrate Chk2-interacting Zn2+ finger (ASCIZ) Is a bi-functional transcriptional activator and feedback sensor in the regulation of dynein light chain (DYNLL1) expression."],"pubmed_authors":["Jörg Heierhorst","Jurado S, Conlan LA, Baker EK, Ng JL, Tenis N, Hoch NC, Gleeson K, Smeets M, Izon D, Heierhorst J"],"additional_accession":[]},"is_claimable":false,"name":"Gene expression changes in primary mouse fibroblasts in the absence of ASCIZ","description":"In order to identify potential transcriptional targets of ASCIZ, we performed a microarray analysis of three wildltype and ASCIZ(ATMIN/ZNF822)-deficient littermate single-passage MEF cultures.  Only 22 and 25 genes were found to be down- or upregulated in the KO, respectively, by >1.5-fold (p<0.05).  The most severely downregulated gene was Dynll1, which was confirmed by Norhtern and Western blot in mulitple embryonic tissues. Primary MEFs were isolated from embryonic day 12.5 embryos (3 wildtype, 3 knockouts), and passaged once before isolation of total RNA.","dates":{"release":"2011-12-21T00:00:00Z","modification":"2023-08-16T03:13:29.817Z","creation":"2022-02-23T15:40:50.005Z"},"accession":"E-GEOD-30417","cross_references":{"GEO":["GSE30417"],"pubmed":["22167198"],"EFO":["EFO_0002768"],"doi":["10.1074/jbc.M111.306019"]}}