biostudies-arrayexpressJörg HeierhorstMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-30417In order to identify potential transcriptional targets of ASCIZ, we performed a microarray analysis of three wildltype and ASCIZ(ATMIN/ZNF822)-deficient littermate single-passage MEF cultures. Only 22 and 25 genes were found to be down- or upregulated in the KO, respectively, by >1.5-fold (p<0.05). The most severely downregulated gene was Dynll1, which was confirmed by Norhtern and Western blot in mulitple embryonic tissues. Primary MEFs were isolated from embryonic day 12.5 embryos (3 wildtype, 3 knockouts), and passaged once before isolation of total RNA.biostudies-arrayexpressGrowth Protocol - 48 hours Dulbecco's Modified Eagle's Medium 10% fetal bovine serum in 10-cm dish, then transfer to 15-cm dish in same mediumHybridization - Standard Illumina hybridization protocolNucleic Acid Extraction - Total RNA was extracted using Rneasy Mini kit (Qiagen).Labeling - Biotinylation using Ambion Total Prep RNA Amplification Kit (Cat. No. IL1791).MIAME ScoreOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsFeature Extraction - All samples were normalised by quantile normalisation method using lumi package in R statistical software.Assay Data Transformation - ID_REF = <br>VALUE = quantile normalizedImage Adquisition - Standard Illumina scanning protocolMetabolomicsUnknownTranscriptomicsGenomicsProteomicsThe highly conserved DYNLL1 (LC8) protein was originally discovered as a light chain of the dynein motor complex, but is increasingly emerging as a sequence-specific regulator of protein dimerization with hundreds of targets and wide-ranging cellular functions. Despite its important roles, DYNLL1's own regulation remains poorly understood. Here we identify ASCIZ (ATMIN/ZNF822), an essential Zn(2+) finger protein with dual roles in the DNA base damage response and as a developmental transcription factor, as a conserved regulator of Dynll1 gene expression. DYNLL1 levels are reduced by ∼10-fold in the absence of ASCIZ in human, mouse and chicken cells. ASCIZ binds directly to the Dynll1 promoter and regulates its activity in a Zn(2+) finger-dependent manner. DYNLL1 protein in turn interacts wtranscription profiling by arrayMus musculusATM substrate Chk2-interacting Zn2+ finger (ASCIZ) Is a bi-functional transcriptional activator and feedback sensor in the regulation of dynein light chain (DYNLL1) expression.Jörg HeierhorstJurado S, Conlan LA, Baker EK, Ng JL, Tenis N, Hoch NC, Gleeson K, Smeets M, Izon D, Heierhorst JfalseGene expression changes in primary mouse fibroblasts in the absence of ASCIZIn order to identify potential transcriptional targets of ASCIZ, we performed a microarray analysis of three wildltype and ASCIZ(ATMIN/ZNF822)-deficient littermate single-passage MEF cultures. Only 22 and 25 genes were found to be down- or upregulated in the KO, respectively, by >1.5-fold (p<0.05). The most severely downregulated gene was Dynll1, which was confirmed by Norhtern and Western blot in mulitple embryonic tissues. Primary MEFs were isolated from embryonic day 12.5 embryos (3 wildtype, 3 knockouts), and passaged once before isolation of total RNA.2011-12-21T00:00:00Z2023-08-16T03:13:29.817Z2022-02-23T15:40:50.005ZE-GEOD-30417GSE3041722167198EFO_000276810.1074/jbc.M111.306019