{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Mark Chong"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-30581"],"description":["Here we describe microRNA profiling of a single differentiation pathway from the stem cell through to terminally differentated mature cells. Populations corresponding to distinct stages in T lymphocyte development, from the hematopoietic stem cell-enriched Lin-Sca+Kit+ population through to mature CD4+ and CD8+ T cells were FACS-sorted to purity from the bone marrow and thymus of C57BL/6 mice. Total RNA was extract from each population from which microRNA sequencing libraries were constructed."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - 19-24nt small RNAs fractionated by gel electrophoresis. Adaptors were ligated only to species with 5' phosphate and 3' hydroxyl"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"pubmed_abstract":["By disrupting microRNA (miRNA) biogenesis, we previously showed that this pathway is critical for the differentiation and function of T cells. Although various cloning studies have shown that many miRNAs are expressed during T cell development, and in a dynamic manner, it was unclear how comprehensive these earlier analyses were. We therefore decided to profile miRNA expression by next generation sequencing. Furthermore, we profiled miRNA expression starting from the hematopoietic stem cell. This analysis revealed that miRNA expression during T cell development is extremely dynamic, with 645 miRNAs sequenced, and the expression of some varying by as much as 3 orders of magnitude. Furthermore, changes in precursor processing led to altered mature miRNA sequences. We also analyzed the struct"],"study_type":["transcription profiling by array"],"species":["Mus musculus"],"pubmed_title":["Dynamic microRNA gene transcription and processing during T cell development."],"pubmed_authors":["Mark Chong","Kirigin FF, Lindstedt K, Sellars M, Ciofani M, Low SL, Jones L, Bell F, Pauli F, Bonneau R, Myers RM, Littman DR, Chong MM"],"additional_accession":[]},"is_claimable":false,"name":"MicroRNA profiling of murine T lymphopoiesis","description":"Here we describe microRNA profiling of a single differentiation pathway from the stem cell through to terminally differentated mature cells. Populations corresponding to distinct stages in T lymphocyte development, from the hematopoietic stem cell-enriched Lin-Sca+Kit+ population through to mature CD4+ and CD8+ T cells were FACS-sorted to purity from the bone marrow and thymus of C57BL/6 mice. Total RNA was extract from each population from which microRNA sequencing libraries were constructed.","dates":{"release":"2012-03-14T00:00:00Z","modification":"2023-08-16T04:03:33.625Z","creation":"2021-09-28T09:43:30Z"},"accession":"E-GEOD-30581","cross_references":{"pubmed":["22379031"],"ENA":["SRP007487"],"EFO":["EFO_0002768"],"doi":["10.4049/jimmunol.1103175"]}}