{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Markus Krohn"],"study_type":["transcription profiling by array"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-30762"],"description":["Comparison between APPPS1-FVB and APPPS1-FVBxABCC1ko mice Mouse brain expression analysis"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Growth Protocol - normal mouse growth","Hybridization - Array hybridization was performed according to the supplier’s instructions using the “Ambion WT Expression Kit” (Ambion, Applied Biosystems, Invitrogen) plus the “GeneChip WT Terminal Labeling and Hybridization Kit” and the “GeneChip Sample Cleanup Module” (Affymetrix). For signal enhancement, an antibody amplification was carried out using a biotinylated antistreptavidin antibody (Vector Laboratories.), which was cross-linked by goat IgG (Sigma) followed by a second staining with streptavidin-phycoerythrin conjugate (Molecular Probes, Invitrogen).","Labeling - GeneChip WT Terminal Labeling and Hybridization Kit” and the “GeneChip Sample Cleanup Module” (Affymetrix)","Nucleic Acid Extraction - Trizol extraction of total RNA was performed according to the manufacturer's instructions."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","Additional Files","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Johannes Steffen","Toni Schumacher","Jens Pahnke","Anne-Sophie Plath","Jörg Gsponer","Katja Scheffler","Felix Winter","Thomas Brüning","Anke Schmidt","Markus Krohn","Franziska Alfen","Jacqueline Hofrichter","Jan Stenzel","Andreas Wree","Katja Rateitschak","Cathleen Lange","Lary Walker"],"data_protocol":["Image Adquisition - G7 upgraded GeneChip Scanner 3000 (Affymetrix) at 0.7 micron resolution","Feature Extraction - The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. Scaled Log2.","Assay Data Transformation - ID_REF = <br>VALUE = MAS5.0 signal intensity"],"additional_accession":[]},"is_claimable":false,"name":"Mouse expression analysis","description":"Comparison between APPPS1-FVB and APPPS1-FVBxABCC1ko mice Mouse brain expression analysis","dates":{"release":"2011-07-18T00:00:00Z","modification":"2022-11-26T14:47:21.899Z","creation":"2021-10-01T17:46:48Z"},"accession":"E-GEOD-30762","cross_references":{"GEO":["GSE30762"],"EFO":["EFO_0002768"]}}