{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Anna Sanecka"],"study_type":["transcription profiling by array"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-31285"],"description":["Differential gene expression upon overexpression of the active form of the transcription factor LUMAN in mouse dendritic cell line D2SC/1 retroviral overexpression of the active form of LUMAN, as a control GFP overexpression was used, two time points = 6h and 18h from the infection start point, time point zero=untreated D2SC/1 cells"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Labeling - GeneChip WT Terminal Labeling Kit","Hybridization - 17 hours at 45 degrees in Affymetrix hybridization oven at 60 rpm,washing and staining in  Fluidics station 450 using stain cocktails: SAPE and antibody solution, (protocol FS450_0001)","Growth Protocol - D2SC/1 cells were cultured in IMDM supplemented with 5% heat-inactivated FCS and 0.5% antibiotic-antimycotic.","Sample Processing - For transduction, D2SC/1 cells were cultured on retronectin-coated 35 mm Petri dishes together with 2 ml of thawed virus containing supernatant for indicated time and then washed with PBS","Sample Processing - For transduction,D2SC/1 cells were cultured on retronectin-coated 35 mm Petri dishes together with 2 ml of thawed virus containing supernatant for indicated time and then washed with PBS","Nucleic Acid Extraction - Rneasy column purification"],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Maaike Looman","Joris Veltman","Maaike van Hout-Kuijer","Marleen Ansems","Dagmar Eleveld-Trancikova","Gosse Adema","Anna Sanecka","Bastiaan Jansen","Christian Gilissen"],"data_protocol":["Image Adquisition - GeneChip scanner 3000 7G","Assay Data Transformation - ID_REF = <br>VALUE = RMA + GC correction by Partek software","Feature Extraction - RMA + GC correction by Partek software"],"additional_accession":[]},"is_claimable":false,"name":"Target genes of LUMAN in dendritic cells","description":"Differential gene expression upon overexpression of the active form of the transcription factor LUMAN in mouse dendritic cell line D2SC/1 retroviral overexpression of the active form of LUMAN, as a control GFP overexpression was used, two time points = 6h and 18h from the infection start point, time point zero=untreated D2SC/1 cells","dates":{"release":"2011-12-31T00:00:00Z","modification":"2023-08-13T16:49:32.971Z","creation":"2022-02-02T13:47:32.741Z"},"accession":"E-GEOD-31285","cross_references":{"GEO":["GSE31285"],"EFO":["EFO_0002768"]}}