biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsAnna Saneckatranscription profiling by arrayMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-31285Differential gene expression upon overexpression of the active form of the transcription factor LUMAN in mouse dendritic cell line D2SC/1 retroviral overexpression of the active form of LUMAN, as a control GFP overexpression was used, two time points = 6h and 18h from the infection start point, time point zero=untreated D2SC/1 cellsbiostudies-arrayexpressLabeling - GeneChip WT Terminal Labeling KitHybridization - 17 hours at 45 degrees in Affymetrix hybridization oven at 60 rpm,washing and staining in Fluidics station 450 using stain cocktails: SAPE and antibody solution, (protocol FS450_0001)Growth Protocol - D2SC/1 cells were cultured in IMDM supplemented with 5% heat-inactivated FCS and 0.5% antibiotic-antimycotic.Sample Processing - For transduction, D2SC/1 cells were cultured on retronectin-coated 35 mm Petri dishes together with 2 ml of thawed virus containing supernatant for indicated time and then washed with PBSSample Processing - For transduction,D2SC/1 cells were cultured on retronectin-coated 35 mm Petri dishes together with 2 ml of thawed virus containing supernatant for indicated time and then washed with PBSNucleic Acid Extraction - Rneasy column purificationMIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsMaaike LoomanJoris VeltmanMaaike van Hout-KuijerMarleen AnsemsDagmar Eleveld-TrancikovaGosse AdemaAnna SaneckaBastiaan JansenChristian GilissenImage Adquisition - GeneChip scanner 3000 7GAssay Data Transformation - ID_REF = <br>VALUE = RMA + GC correction by Partek softwareFeature Extraction - RMA + GC correction by Partek softwarefalseTarget genes of LUMAN in dendritic cellsDifferential gene expression upon overexpression of the active form of the transcription factor LUMAN in mouse dendritic cell line D2SC/1 retroviral overexpression of the active form of LUMAN, as a control GFP overexpression was used, two time points = 6h and 18h from the infection start point, time point zero=untreated D2SC/1 cells2011-12-31T00:00:00Z2023-08-13T16:49:32.971Z2022-02-02T13:47:32.741ZE-GEOD-31285GSE31285EFO_0002768