biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsDaniel Wagnertranscription profiling by arraySchmidtea mediterraneaSchmidtea mediterraneahttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-34969The goal of the study was to identify genes expressed in the proliferative cell population (neoblasts) of adult planarians. Proliferating cells are rapidly and specifically eliminated following lethal irradiation. Genes expressed by neoblasts, therefore, were expected to show decreased level of expression following 6,000 rads, relative to control animals. Total RNA was collected from control (unirradiated) animals and from animals 6, 12, 24, and 48 hours following 6,000 rads irradiation. Between three and five replicates were used for each samplebiostudies-arrayexpressGrowth Protocol - Animals were maintained at 20 degrees celsius.Sample Processing - RNA was harvested from control animals and from animals after exposure to 6,000 rads ionizing radiationLabeling - Cy3 and Cy5-labeled cRNA was prepared using a QuickAmp labeling kit (Agilent) starting with 1μg total RNANucleic Acid Extraction - RNA was extracted using the standard Trizol methodHybridization - Agilent custom planarian 4x44,000 expression arrays were hybridized according manufacturer instructions and scanned using an Agilent DNA microarray scanner.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataAdditional FilesMAGE-TAB FilesArray DesignsPeter ReddienDaniel WagnerFeature Extraction - Agilent two-color arrays were within-array normalized by loess, followed by between-array quantile normalization of average intensities across channels (Aquantile).Assay Data Transformation - ID_REF = <br>VALUE = log2 (irradiated/ control) after loess and Aquantile normalizationImage Adquisition - Array images were quantified and statistical significance of differential expression was calculated using Agilent’s Feature Extraction Image Analysis software with the default two-color gene expression protocol.falseGene Expression Profiles of Planarians after 6,000 Rads IrradiationThe goal of the study was to identify genes expressed in the proliferative cell population (neoblasts) of adult planarians. Proliferating cells are rapidly and specifically eliminated following lethal irradiation. Genes expressed by neoblasts, therefore, were expected to show decreased level of expression following 6,000 rads, relative to control animals. Total RNA was collected from control (unirradiated) animals and from animals 6, 12, 24, and 48 hours following 6,000 rads irradiation. Between three and five replicates were used for each sample2012-01-10T00:00:00Z2022-11-23T15:05:32.665Z2022-03-10T08:04:16.511ZE-GEOD-34969GSE34969EFO_0002768