biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsTrang Vutranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-34987Analysis of differentially expressed genes in response to the LXR agonist GW3965 in the MCF-7, T-47D, SK-BR-3, and MDA-MB-231 breast cancer cell lines. It was previously reported that GW3965 has antiproliferative effects on these 4 different breast cancer cell lines. In the present study, we additionally determine the effects of the LXR ligand on breast cancer cells and determine their mechanism of action in reducing cell proliferation. Total RNA obtained from 4 different breast cancer cell lines (MCF-7, T-47D, SK-BR-3, MDA-MB-231) grown in culture treated with ethanol (control) or GW3965 (GW-treated, experimental) for 48 hours. Triplicates were performed.biostudies-arrayexpressSample Treatment - Cells were treated with 10 uM synthetic LXR agonist GW3965 for 48 hours prior to harvest and RNA isolation.Nucleic Acid Extraction - RNA was extracted using the EZNA Total RNA Kit I from Omega.Labeling - cRNA were prepared and labeled with Cy3 dye using the Illumina TotalPrep-96RNA Amplification Kit and Illumina Whole-Genome Gene Expression Direct Hybridisation Kit.Growth Protocol - Breast cancer cell lines were grown in respective media with 5-10% FBS.Hybridization - Standard Illumina hybridization protocol.Scaning - Standard Illumina scanning protocol.MIAME ScoreOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsChin LinTrang VuData Transformation - Data was normalized and background-corrected using the R Program, lumi package. Comparisons were done with the R program limma package. For our analyses, only probes that align to a single gene on the human genome as defined by Ensembl were used in the final data set. ID_REF = VALUE = Quantile-normalized signal Detection Pval =falseGenome-wide analysis of GW3965-responsive genes in 4 different breast cancer cell lines, MCF-7, T-47D, SK-BR-3, and MDA-MB-231Analysis of differentially expressed genes in response to the LXR agonist GW3965 in the MCF-7, T-47D, SK-BR-3, and MDA-MB-231 breast cancer cell lines. It was previously reported that GW3965 has antiproliferative effects on these 4 different breast cancer cell lines. In the present study, we additionally determine the effects of the LXR ligand on breast cancer cells and determine their mechanism of action in reducing cell proliferation. Total RNA obtained from 4 different breast cancer cell lines (MCF-7, T-47D, SK-BR-3, MDA-MB-231) grown in culture treated with ethanol (control) or GW3965 (GW-treated, experimental) for 48 hours. Triplicates were performed.2013-07-17T00:00:00Z2023-08-19T14:34:16.604Z2022-02-03T08:44:19.382ZE-GEOD-34987GSE34987EFO_0002768