{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Johan Skog"],"study_type":["transcription profiling by array"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-35444"],"description":["Primary glioblastoma cells and primary malignant melanoma cells were grown in vitro (less than 15 passages). Exosomes/microvesicles were isolated from the media supernatant at the same time as RNA was extracted from the cells. The goal of the study was to compare the gene expression profiles in exosomes/microvesicles compared to the cells that release them. Analysis of tumor cell RNA (glioma and melanoma) and their corresponding microvesicles."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Processing - Cells were washed and fresh media was added. Exosomes/microvesicles were harvested after 48 hrs. At the same time, the cells were harvested for RNA extraction. Exosomes/microvesicles were isolated by differential centrifugation according to previously published protocol (Skog J et al. Nat Cell Biol 2008)","Labeling - Standard Illumina labelling protocol","Nucleic Acid Extraction - RNA was extracted with the Ambion miRNeasy RNA extraction kit, followed by quality assessment on a Bioanalyzer RNA pico chip.","Hybridization - Standard Illumina hybridization protocol.","Growth Protocol - DMEM medium supplemented with 5% microvesicle free fetal bovine serum."],"figure_sub":["MIAME Score","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Jian-Bing Fan","Johan Skog"],"data_protocol":["Feature Extraction - Raw data was analyzed using GenomeStudio v1.1","Assay Data Transformation - ID_REF = <br>VALUE = AVG_Signal","Image Adquisition - Standard Illumina scanning protocol"],"additional_accession":[]},"is_claimable":false,"name":"Analysis of tumor cell RNA (glioma and melanoma) and their corresponding microvesicles","description":"Primary glioblastoma cells and primary malignant melanoma cells were grown in vitro (less than 15 passages). Exosomes/microvesicles were isolated from the media supernatant at the same time as RNA was extracted from the cells. The goal of the study was to compare the gene expression profiles in exosomes/microvesicles compared to the cells that release them. Analysis of tumor cell RNA (glioma and melanoma) and their corresponding microvesicles.","dates":{"release":"2012-01-31T00:00:00Z","modification":"2023-08-19T18:25:38.495Z","creation":"2022-02-23T18:58:09.962Z"},"accession":"E-GEOD-35444","cross_references":{"GEO":["GSE35444"],"EFO":["EFO_0002768"]}}