biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsBing Hucomparative genomic hybridization by arrayMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-36464Skin tumor formed in the ColIa2 Cre x RBP-Jkappa loxp/loxp mice was analyzed for genomic changes by comparing with normal tissues from the same mice. Genomic DNA were isolated from each individual tumor and compared with the DNA extracted from the brain tissue in the same mouse.biostudies-arrayexpressNucleic Acid Extraction - Excised skin tumors were minced into small pieces and digested in 1% Collagenase I (Sigma; 37oC for 1 hour) for removal of stromal tissue. The resulting suspension was filtered through a 40mm mesh and epithelial fragments were used for DNA extraction, using a Wizard® Genomic DNA Purification Kit (Promega).Labeling - Tumor DNA samples were labelled with Cy3 and hybridized to Nimblegen mouse CGH arrays together with Cy5-labelled normal brain DNA extracted from the same mice.Hybridization - Arrays contained 2.1 million probes across the whole genome based on the MM9 genome build. CGH was according to Nimblegen protocolsMIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsBing HuGian DottoImage Adquisition - Scanning with an Agilent G2565BA Microarray Scanner was followed by imageAssay Data Transformation - ID_REF = <br>VALUE = Log ratio of tumor versus normal tissueFeature Extraction - Data were processed using Nimblescan software v.2.5.falseStudy on the genomic changes in ColIa2 Cre x RBP-Jkappa loxp/loxp miceSkin tumor formed in the ColIa2 Cre x RBP-Jkappa loxp/loxp mice was analyzed for genomic changes by comparing with normal tissues from the same mice. Genomic DNA were isolated from each individual tumor and compared with the DNA extracted from the brain tissue in the same mouse.2012-04-30T00:00:00Z2023-08-20T06:23:32.931Z2022-03-04T16:59:26.275ZE-GEOD-36464GSE36464EFO_0000749