{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["julien colombani"],"study_type":["transcription profiling by array"],"organism":["Drosophila melanogaster"],"species":["Drosophila melanogaster"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-36862"],"description":["Classical regeneration experiments in insects have demonstrated an important role for imaginal tissues (also called discs, the larval tissues that give rise to the adult appendages) in coupling tissue growth, maturation and patterning during development We used the rotund-Gal4 driver (Rn>) for disc-targeted silencing of the avalanche gene (avl; Rn>avl-RNAi), encoding a syntaxin that functions in the early endocytic machinery (H. Lu, D. Bilder, Nat Cell Biol 7, 1232; Dec, 2005). Rn>avl-RNAi discs reach near to normal size after 5 days of development, and then undergo unrestricted neoplastic growth. We were interested in identifying genes showing differential expression profiles in control and in neoplastic growth. We identified dilp8 as one of the most differentially expressed gene in control and Rn>avl-RNAi discs. Abstract from the associated publication: Little is known about how organ growth is monitored and coordinated with the developmental timing in complex organisms. In insects, impairment of larval tissue growth delays growth and morphogenesis, revealing a coupling mechanism.  We carried out a genetic screen in Drosophila to identify molecules expressed by growing tissues participating in this coupling and identified dilp8 as a gene whose silencing rescues the developmental delay induced by abnormally growing tissues. dilp8 is highly induced in conditions where growth impairment produces a developmental delay. dilp8 encodes a peptide whose expression and secretion is sufficient to delay metamorphosis without affecting tissue integrity. We propose that dilp8 is a secreted signal that coordinates the growth status of tissues with developmental timing. RNA were extracted from wing discs of either Rn>+ or Rn>Avl RNAi staged third larval instar at 92, 116 or 168 hours AED (After Egg Deposition) using Qiagen RNeasy lipid tissue microkit according to the manufacturer protocol. Three separate samples were collected for each time point."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Processing - Wing imaginal discs were dissected on ice and fast frozen in liquid N2.","Nucleic Acid Extraction - Qiazol extraction of total RNA was performed according to the manufacturer's instructions for Qiagen RNeasy lipid tissue microkit. RNA were analysed by electrophoresis with a Agilent Bioanalyzer.","Growth Protocol - 2 hours egg collections of Rn>+ or Rn>Avl RNAi flies and development until 92, 116, or 168 hours at 25C .","Labeling - Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).","Hybridization - Following fragmentation, cRNA were hybridized on Affimetrix Drosophila _2 Gene Array. GeneChips were washed and stained in the GeneChip Affymetrix Station."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","Additional Files","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Julien Colombani","Ditte Andersen","julien colombani","Pierre Leopold"],"data_protocol":["Feature Extraction - The data were analyzed with Partek®GSv.6.5) and using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set. The robustness has been validated by independent methods (nonparametric SAM by Tibshirani and EBS form R/Bioconductor:limma)","Image Adquisition - GeneChips were scanned using the GeneChip scanner 3000 7G.","Assay Data Transformation - ID_REF = <br>VALUE = Log2 GC-RMA signal"],"additional_accession":[]},"is_claimable":false,"name":"Genes showing differential expression profiles in control and in neoplastic growth using drosophila wing imaginal tissues","description":"Classical regeneration experiments in insects have demonstrated an important role for imaginal tissues (also called discs, the larval tissues that give rise to the adult appendages) in coupling tissue growth, maturation and patterning during development We used the rotund-Gal4 driver (Rn>) for disc-targeted silencing of the avalanche gene (avl; Rn>avl-RNAi), encoding a syntaxin that functions in the early endocytic machinery (H. Lu, D. Bilder, Nat Cell Biol 7, 1232; Dec, 2005). Rn>avl-RNAi discs reach near to normal size after 5 days of development, and then undergo unrestricted neoplastic growth. We were interested in identifying genes showing differential expression profiles in control and in neoplastic growth. We identified dilp8 as one of the most differentially expressed gene in control and Rn>avl-RNAi discs. Abstract from the associated publication: Little is known about how organ growth is monitored and coordinated with the developmental timing in complex organisms. In insects, impairment of larval tissue growth delays growth and morphogenesis, revealing a coupling mechanism.  We carried out a genetic screen in Drosophila to identify molecules expressed by growing tissues participating in this coupling and identified dilp8 as a gene whose silencing rescues the developmental delay induced by abnormally growing tissues. dilp8 is highly induced in conditions where growth impairment produces a developmental delay. dilp8 encodes a peptide whose expression and secretion is sufficient to delay metamorphosis without affecting tissue integrity. We propose that dilp8 is a secreted signal that coordinates the growth status of tissues with developmental timing. RNA were extracted from wing discs of either Rn>+ or Rn>Avl RNAi staged third larval instar at 92, 116 or 168 hours AED (After Egg Deposition) using Qiagen RNeasy lipid tissue microkit according to the manufacturer protocol. Three separate samples were collected for each time point.","dates":{"release":"2012-03-27T00:00:00Z","modification":"2022-11-24T00:47:51.999Z","creation":"2022-02-07T21:26:58.15Z"},"accession":"E-GEOD-36862","cross_references":{"GEO":["GSE36862"],"EFO":["EFO_0002768"]}}