biostudies-arrayexpressSean DavisHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-4406CD4+ T-cells isolated from three normal individuals and GM6990 cell lines (three biological replicates) are compared Experiment Overall Design: 8 arrays comparing CD4+ T-cells and GM6990 lymphoblastoid cell linesbiostudies-arrayexpressNucleic Acid Extraction - CD4+ T-cells purified using negative selectionNucleic Acid Extraction - GM6990Labeling - See manuscriptMIAME ScoreOrganizationAssays and DataMAGE-TAB FilesArray DesignsMetabolomicsUnknownTranscriptomicsGenomicsProteomicsMapping DNase I hypersensitive sites is an accurate method of identifying the location of gene regulatory elements, including promoters, enhancers, silencers and locus control regions. Although Southern blots are the traditional method of identifying DNase I hypersensitive sites, the conventional manual method is not readily scalable to studying large chromosomal regions, much less the entire genome. Here we describe DNase-chip, an approach that can rapidly identify DNase I hypersensitive sites for any region of interest, or potentially for the entire genome, by using tiled microarrays. We used DNase-chip to identify DNase I hypersensitive sites accurately from a representative 1% of the human genome in both primary and immortalized cell types. We found that although most DNase I hypersenstranscription profiling by arrayHomo sapiensDNase-chip: a high-resolution method to identify DNase I hypersensitive sites using tiled microarrays.Sean DavisGregory E Crawford, Sean Davis, Peter C Scacheri, Gabriel Renaud, Mohamad J Halawi, Michael R Erdos, Roland Green, Paul S Meltzer, Tyra G Wolfsberg, Francis S CollinsfalseTranscription profiling of human CD4+ T-cells and GM6990 lymphoblastoid cell linesCD4+ T-cells isolated from three normal individuals and GM6990 cell lines (three biological replicates) are compared Experiment Overall Design: 8 arrays comparing CD4+ T-cells and GM6990 lymphoblastoid cell lines2008-06-13T00:00:00Z2023-08-30T04:52:43.707Z2021-10-06T16:03:14ZE-GEOD-4406GSE440616791207EFO_000276816791207