{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Lester Kobzik"],"study_type":["transcription profiling by array"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-44678"],"description":["This study aims to demonstrate the link between epigenome-wide methylation aberrations at birth and genomic transcriptional changes upon allergen sensitization that occur in the neonatal dendritic cells (DC) due to maternal asthma. In an in vivo model reproducing human epidemiology findings, maternal but not paternal asthma predisposes the neonate to increased asthma risk, the effect is allergen-independent and is not genetic or environmental. Earlier we demonstrated that neonates of asthmatic mothers are born with a functional skew in splenic DCs that mediates the early-life asthma origin. These allergen-naive cells convey allergy responses to normal recipients, however minimal to no transcriptional or phenotypic changes were found to explain the functional pro-allergic alterations. In this study we profiled both allergen-naïve dendritic cells, and cells after allergen sensitization in vivo.    We found that while allergen-naive DCs from asthma-at-risk neonates have minimal transcriptional change compared to controls, upon allergen sensitization, multiple genes with pre-existing epigenetic alterations show significant transcriptional change.   . 24 samples from 2 batches, 3-4 replicates in each of 4 groups"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Scaning - Standard Affymetrix procedure","Hybridization - Standard Affymetrix procedure","Sample Treatment - Neonate mice were either naïve, or sensitized with a single i.p. injection of 5 ug of OVA with 1 mg alum in 0.1 PBS at day 3.","Labeling - Standard Affymetrix procedure","Nucleic Acid Extraction - Dendritic cells were isolated from splenocyte suspension using magnetic cell separation (Miltenyi). Genomic DNA was isolated using Qiagen procedure.","Growth Protocol - Maternal sensitization is achieved by initial i.p. injections of 5 μg OVA with 1 mg alum in 0.1 ml PBS into mice at 3 and 7 days of age. After weaning, female mice are exposed to aerosols of allergen (3% (w/v) OVA (grade V, Sigma-Aldrich) in PBS, pH 7.4) for 10 min on 3 consecutive days at 4, 8, and 12 wk of age, and then mated with normal male mice. The aerosol exposure is performed within individual compartments of a mouse pie chamber (Braintree Scientific, Braintree, MA) using a Pari IS2 nebulizer (Sun Medical Supply, Kansas City, KS) connected to air compressor (PulmoAID; DeVilbiss, Somerset, PA). These female mice were shown to consistently have strong allergic airway inflammation and evidence of Th2 polarization."],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Lester Kobzik","Lyudmila Mikhaylova","Alexey Fedulov"],"data_protocol":["Data Transformation - RMA normalization with background adjustment ID_REF =  VALUE = RMA natural scale"],"additional_accession":[]},"is_claimable":false,"name":"Effect of allergen sensitization on gene expression in dendritic cells from neonates of asthmatic vs control mothers","description":"This study aims to demonstrate the link between epigenome-wide methylation aberrations at birth and genomic transcriptional changes upon allergen sensitization that occur in the neonatal dendritic cells (DC) due to maternal asthma. In an in vivo model reproducing human epidemiology findings, maternal but not paternal asthma predisposes the neonate to increased asthma risk, the effect is allergen-independent and is not genetic or environmental. Earlier we demonstrated that neonates of asthmatic mothers are born with a functional skew in splenic DCs that mediates the early-life asthma origin. These allergen-naive cells convey allergy responses to normal recipients, however minimal to no transcriptional or phenotypic changes were found to explain the functional pro-allergic alterations. In this study we profiled both allergen-naïve dendritic cells, and cells after allergen sensitization in vivo.    We found that while allergen-naive DCs from asthma-at-risk neonates have minimal transcriptional change compared to controls, upon allergen sensitization, multiple genes with pre-existing epigenetic alterations show significant transcriptional change.   . 24 samples from 2 batches, 3-4 replicates in each of 4 groups","dates":{"release":"2013-06-01T00:00:00Z","modification":"2023-08-26T23:01:44.495Z","creation":"2022-03-09T02:47:42.771Z"},"accession":"E-GEOD-44678","cross_references":{"GEO":["GSE44678"],"EFO":["EFO_0002768"]}}