biostudies-arrayexpress00560UnknownTranscriptomicsGenomicsProteomicsProfessor Anthony Segaltranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-48634Background and aims: Mucosal abnormalities are potentially important in the primary pathogenesis of ulcerative colitis (UC). We investigated the mucosal transcriptomic expression profiles of biopsies from patients with UC and healthy controls (HC), taken from macroscopically non-inflamed tissue from the terminal ileum and three colonic locations with the objective of identifying abnormal molecules that might be involved in disease development. Methods: Whole-genome transcriptional analysis was performed on intestinal biopsies taken from 24 UC, 26 HC and 14 patients with CrohnM-bM-^@M-^Ys disease. Differential gene expression analysis was performed at each tissue location separately and results were then meta-analysed using FisherM-bM-^@M-^Ys method. Significantly differentially expressed genes were validated using qPCR. Gene location within the colon was determined using immunohistochemistry, subcellular fractionation, electron and confocal microscopy. DNA methylation was quantified by pyrosequencing. Results: Seven probes were abnormally expressed throughout the colon in UC patients with Family with sequence similarity member 5 C (FAM5C) being the most significantly underexpressed. Attenuated expression of FAM5C in UC was independent of inflammation, unrelated to phenotype or treatment, and remained low at rebiopsy approximately 23 months later. FAM5C is localised to the brush border of the colonic epithelium and expression is influenced by DNA methylation within its promoter. Conclusion: Genome-wide expression analysis of non-inflamed mucosal biopsies from UC patients identified FAM5C as significantly under-expressed throughout the colon in a major sub-set of patients with UC. Low levels of this gene could predispose to or contribute to the maintenance of the characteristic mucosal inflammation seen in this condition. Total RNA was extracted from the intestinal biopsies taken from macroscopically normal mucosa in the rectum, descending colon, ascending colon and terminal ileum in clinically quiescent Ulcerative colitis and Crohn's disease patients and compared to healthy controls. Normalized data for 26,261 probes out of 47,323 only. Criteria for inclusion not specified. The non-normalized matrix contains the complete non-normalized data for all probes.biostudies-arrayexpressLabeling - 750 ng of biotin-labelled cRNA (150 ng/M-NM-<l) was hybridised to Illumina HumanHT-12v4 beadarrays (Illumina, CA, USA) for 16 hours at 58M-0C.Nucleic Acid Extraction - Biopsies (~25 mg) were lysed in a 300 M-5l RNeasy Fibrous Tissue kit (Qiagen, Hilden, Germany) RLT buffer and 0.14 M M-NM-2-mercaptoethanol (M-NM-2-ME) (Sigma-Aldrich, St. Louis, USA), and then homogenised by centrifugation at 10,000 g through a Qiashredder column (Qiagen). Protein was removed by incubation for 10 minutes at 55M-0C with 10 M-5l Proteinase K (20 mg/ml) (>600 mAU/ml) (Qiagen). Total RNA was extracted on RNeasy Mini spin columns and DNA was removed with RNase free DNase digestion (Qiagen). Samples were included in the microarray experiment if they had an optical density ratio reading of 1.8-2.0 OD260/OD280 and >1.8 OD260/OD230 as well as a RNA concentration of greater than 50 ng/M-NM-<l. For each biopsy sample, 500 ng of total RNA was amplified and purified using the Illumina TotalPrep-96 RNA Amplification kit (AmbionM-., Life TechnologiesM-bM-^DM-", CA, USA).Hybridization - 750 ng of biotin-labelled cRNA (150 ng/M-NM-<l) was hybridised to Illumina HumanHT-12v4 beadarrays (Illumina, CA, USA) for 16 hours at 58M-0C.Scaning - Following hybridisation, beadarrays were washed and stained with streptavidin-Cy3 (GE Healthcare, UK), scanned using the Beadarray reader, and processed using Genome Studio software (Illumina).MIAME ScoreOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsRoser VegaMarco NovelliProfessor Anthony SegalMark TurmaineStephan BeckDahmane OukrifJenny DunneJennifer PatersonStuart BloomManuel Rodriguez-JustoGavin SewellAndrew SmithAnthony SegalNuala O'SheaAdam LevinePaul GuilhamonPhilip SmithData Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B19 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B27 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B157 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B114 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B165 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B122 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B10 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B53 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B94 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B3 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B124 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B5 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B167 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B96 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B86 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B140 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B43 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B61 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B35 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B132 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B78 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B106 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B149 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B20 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B25 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B33 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B12 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B17 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B41 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B112 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B98 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B159 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B120 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B1 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B88 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B51 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B130 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B169 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B15 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B104 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B57 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B136 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B144 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B31 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B101 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B110 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B14 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B128 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B73 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B23 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B30 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B152 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B81 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B135 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B49 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B99 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B56 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B127 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B171 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B48 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B102 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B65 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B119 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B22 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B76 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B68 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B84 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B42 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B155 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B7 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B139 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B34 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B163 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B92 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B45 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B116 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B29 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B113 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B108 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B121 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B11 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B50 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B147 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B160 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B37 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B95 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B131 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B52 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B123 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B87 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B44 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B60 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B107 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B148 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B36 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B79 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B105 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B18 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B69 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B26 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B141 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B70 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B115 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B158 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B166 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B4 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B38 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B117 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B46 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B125 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B59 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B146 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B77 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B109 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B62 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B133 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B64 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B143 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B151 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B75 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B72 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B156 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B85 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B80 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B54 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B28 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B67 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B138 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B6 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B164 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B93 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B82 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B9 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B66 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B90 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B161 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B13 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B83 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B137 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B118 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B154 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B40 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B170 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B111 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B153 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B74 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B8 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B145 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B39 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B162 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B91 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B150 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B168 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B89 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B55 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B142 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B71 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B103 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B47 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B21 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B100 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B32 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B126 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B129 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B58 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B2 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B16 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B97 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B24 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B63 Detection Pval =Data Transformation - Expression data for Illumina HumanHT-12v4 beadarrays was normalised after Log2 transformation within Genome Studio (Illumina) using cubic spline normalisation followed by ComBat normalisation for chip effects in R. ID_REF = VALUE = cubic spline normalized (Probes that reached a minimum detection p-value of p<0.01 in at least two biopsies in any location were included in the subsequent analyses (n=26,261 probes) B134 Detection Pval =56falseMucosal transcriptomics implicates under expression of FAM5C in the pathogenesis of ulcerative colitisBackground and aims: Mucosal abnormalities are potentially important in the primary pathogenesis of ulcerative colitis (UC). We investigated the mucosal transcriptomic expression profiles of biopsies from patients with UC and healthy controls (HC), taken from macroscopically non-inflamed tissue from the terminal ileum and three colonic locations with the objective of identifying abnormal molecules that might be involved in disease development. Methods: Whole-genome transcriptional analysis was performed on intestinal biopsies taken from 24 UC, 26 HC and 14 patients with CrohnM-bM-^@M-^Ys disease. Differential gene expression analysis was performed at each tissue location separately and results were then meta-analysed using FisherM-bM-^@M-^Ys method. Significantly differentially expressed genes were validated using qPCR. Gene location within the colon was determined using immunohistochemistry, subcellular fractionation, electron and confocal microscopy. DNA methylation was quantified by pyrosequencing. Results: Seven probes were abnormally expressed throughout the colon in UC patients with Family with sequence similarity member 5 C (FAM5C) being the most significantly underexpressed. Attenuated expression of FAM5C in UC was independent of inflammation, unrelated to phenotype or treatment, and remained low at rebiopsy approximately 23 months later. FAM5C is localised to the brush border of the colonic epithelium and expression is influenced by DNA methylation within its promoter. Conclusion: Genome-wide expression analysis of non-inflamed mucosal biopsies from UC patients identified FAM5C as significantly under-expressed throughout the colon in a major sub-set of patients with UC. Low levels of this gene could predispose to or contribute to the maintenance of the characteristic mucosal inflammation seen in this condition. Total RNA was extracted from the intestinal biopsies taken from macroscopically normal mucosa in the rectum, descending colon, ascending colon and terminal ileum in clinically quiescent Ulcerative colitis and Crohn's disease patients and compared to healthy controls. Normalized data for 26,261 probes out of 47,323 only. Criteria for inclusion not specified. The non-normalized matrix contains the complete non-normalized data for all probes.E-GEOD-48634GSE48634EFO_0002768