{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Katharina Lahl"],"study_type":["transcription profiling by array"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-50380"],"description":["This file contains gene microarray data from subsets of human intestinal dendritic cells, as defined by their expression of CD103 and Sirpa. This will allow for better understanding of human intestinal DC subsets in general and will facilitate translation from findings in the mouse. We analyzed transcript expression data from small intestine DC subsets isolated from de-identified human subject undergoing bariatric surgery."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Scaning - Array scanning was performed by Expression Analysis, Inc. according to the manufacturer's instruction","Hybridization - Samples were hybridized with Affymetrix GeneChip Human Exon 1.0 ST Arrays and scanned by Expression Analysis, Inc.","Labeling - Single-stranded cDNA was generated from amplified cRNA (using Ambion WT Expression Kit) and then fragmented/labeled with the Affymetrix GeneChip WT Terminal Labeling and Controls Kit, performed by Expression Analysis, Inc.","Nucleic Acid Extraction - Total RNA was isolated from sorted human gut DC subsets using Qiagen RNeasy Plus Micro kit following manufacturer’s protocol"],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Katharina Lahl","Payal Watchmaker","John Morton","Eugene Butcher","Mike Lee"],"data_protocol":["Data Transformation - GeneSpring GX 11.5 and Partek Genomic Suite (6.6) were used for processing and analyzing the data. Genespring preprocessing and default normalization (RMA-16) was applied to each dataset. Chp files were generated by Expression Analysis, Inc. ID_REF =  VALUE = Quantification"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptional and functional profiling of human intestinal dendritic cells","description":"This file contains gene microarray data from subsets of human intestinal dendritic cells, as defined by their expression of CD103 and Sirpa. This will allow for better understanding of human intestinal DC subsets in general and will facilitate translation from findings in the mouse. We analyzed transcript expression data from small intestine DC subsets isolated from de-identified human subject undergoing bariatric surgery.","dates":{"release":"2013-11-01T00:00:00Z","modification":"2023-09-02T16:02:09.961Z","creation":"2022-03-15T14:53:07.274Z"},"accession":"E-GEOD-50380","cross_references":{"GEO":["GSE50380"],"EFO":["EFO_0002768"]}}