biostudies-arrayexpressJingjing QiMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-50785Analysis of genes regulated by Shp2 mediated signaling in the small intestine Total RNA from small intestine of 6 villinCre;Shp2fl conditional mutant mice was compared to 6 control micebiostudies-arrayexpressScaning - Standard Illumina scanning protocolLabeling - Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification KitNucleic Acid Extraction - RNA from whole piece of proximal small intestine was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.Hybridization - Standard Illumina hybridization protocolMIAME ScoreOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsData Transformation - The data were quantile normalized with Illumina Genome Studio, data analysis and quality control were carried out using Partek® software. ID_REF = VALUE = quantile normalized Detection Pval =MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIn the development of the mammalian intestine, Notch and Wnt/β-catenin signals control stem cell maintenance and their differentiation into absorptive and secretory cells. Mechanisms that regulate differentiation of progenitors into the three secretory lineages, goblet, paneth, or enteroendocrine cells, are not fully understood. Using conditional mutagenesis in mice, we observed that Shp2-mediated MAPK signaling determines the choice between paneth and goblet cell fates and also affects stem cells, which express the leucine-rich repeat-containing receptor 5 (Lgr5). Ablation of the tyrosine phosphatase Shp2 in the intestinal epithelium reduced MAPK signaling and led to a reduction of goblet cells while promoting paneth cell development. Conversely, conditional mitogen-activated protein kinatranscription profiling by arrayMus musculusShp2/MAPK signaling controls goblet/paneth cell fate decisions in the intestine.Heuberger J, Kosel F, Qi J, Grossmann KS, Rajewsky K, Birchmeier WJingjing QiJulian HeubergerWalter BirchmeierfalseGenome-wide analysis of small intestinal gene expression of control and villinCre:Shp2fl mutant miceAnalysis of genes regulated by Shp2 mediated signaling in the small intestine Total RNA from small intestine of 6 villinCre;Shp2fl conditional mutant mice was compared to 6 control mice2014-02-24T00:00:00Z2023-09-02T20:06:45.098Z2022-03-04T18:53:44.35ZE-GEOD-50785GSE5078524550486EFO_000276810.1073/pnas.1309342111