{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Christopher Sullivan"],"study_type":["transcription profiling by array"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-51520"],"description":["Analysis of gene expression changes in response to TTV-tth8 miRNA Total RNA harvested 30 hours after transfection of HEK293TT cells with recircularized TTV-tth8 genome, miRNA mutant TTV-tth8 genome, SV40 miRNA recombinant TTV-tth8  genome, or pUC vector using Lipofectamine 2000."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Scaning - Standard Illumina scanning protocol","Labeling - Biotinylated cRNA were prepared with the using an Illumina TotalPrep RNA Amplification Kit (Ambion)","Nucleic Acid Extraction - RNA was extracted with PIG-B reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.","Hybridization - Standard Illumina hybridization protocol"],"figure_sub":["MIAME Score","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Christopher Sullivan","Rodney Kincaid"],"data_protocol":["Data Transformation - Images were processed with Beadstudio software and data were normalized using quantile normalization ID_REF =  VALUE = quantile normalized signal Detection Pval =  BEAD_STDERR =  Avg_NBEADS ="],"additional_accession":[]},"is_claimable":false,"name":"Microarray Study to Identify Candidate TTV-tth8 microRNA Targets","description":"Analysis of gene expression changes in response to TTV-tth8 miRNA Total RNA harvested 30 hours after transfection of HEK293TT cells with recircularized TTV-tth8 genome, miRNA mutant TTV-tth8 genome, SV40 miRNA recombinant TTV-tth8  genome, or pUC vector using Lipofectamine 2000.","dates":{"release":"2013-12-02T00:00:00Z","modification":"2023-09-03T08:53:16.626Z","creation":"2022-03-06T23:31:22.382Z"},"accession":"E-GEOD-51520","cross_references":{"GEO":["GSE51520"],"EFO":["EFO_0002768"]}}