biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsChristopher Sullivantranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-51520Analysis of gene expression changes in response to TTV-tth8 miRNA Total RNA harvested 30 hours after transfection of HEK293TT cells with recircularized TTV-tth8 genome, miRNA mutant TTV-tth8 genome, SV40 miRNA recombinant TTV-tth8 genome, or pUC vector using Lipofectamine 2000.biostudies-arrayexpressScaning - Standard Illumina scanning protocolLabeling - Biotinylated cRNA were prepared with the using an Illumina TotalPrep RNA Amplification Kit (Ambion)Nucleic Acid Extraction - RNA was extracted with PIG-B reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.Hybridization - Standard Illumina hybridization protocolMIAME ScoreOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsChristopher SullivanRodney KincaidData Transformation - Images were processed with Beadstudio software and data were normalized using quantile normalization ID_REF = VALUE = quantile normalized signal Detection Pval = BEAD_STDERR = Avg_NBEADS =falseMicroarray Study to Identify Candidate TTV-tth8 microRNA TargetsAnalysis of gene expression changes in response to TTV-tth8 miRNA Total RNA harvested 30 hours after transfection of HEK293TT cells with recircularized TTV-tth8 genome, miRNA mutant TTV-tth8 genome, SV40 miRNA recombinant TTV-tth8 genome, or pUC vector using Lipofectamine 2000.2013-12-02T00:00:00Z2023-09-03T08:53:16.626Z2022-03-06T23:31:22.382ZE-GEOD-51520GSE51520EFO_0002768