{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Peter Sternes"],"study_type":["RNA-seq of non coding RNA"],"organism":["Saccharum sp."],"species":["Saccharum sp."],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-53304"],"description":["We report the deep sequencing of small RNA populations derived from apex, internode 3, internode 6, internode 16, leaf +3, calli and suspesion cells from sugarcane cultivar Q117 Each vegetative tissue was sampled from three independent field grown plants, pooled and sequenced using the GAIIx platform. Calli and suspension cell cultures were sequenced using the HiSeq 2000 platform."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Total RNA was isolated using TRIsure reagent (Bioline) as per the manufacturer's intstructions Libraries were constructed using the TruSeq Small RNA kit (Illumina)"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"pubmed_authors":["Peter Sternes","Richard Moyle"],"data_protocol":["Data Transformation - Sequencing adapter primers (proprietary) were trimmed using UNIX shell scripting Sense and antisense matches to known tRNA, rRNA and chloroplast sequences from Rfam, Genomic tRNA, EMBL release 95 and SILVA rRNA databases were removed using UNIX shell scripting Sequences were counted and displayed as tab delimited files containing a count and the unique sequence per line, using UNIX shell scripting Supplementary_files_format_and_content: Tab delimited text files containing counts of trimmed and filtered small RNA sequences"],"additional_accession":[]},"is_claimable":false,"name":"Deep sequencing reveals divergent expression patterns within the small RNA transcriptomes of cultured and vegetative tissues of sugarcane","description":"We report the deep sequencing of small RNA populations derived from apex, internode 3, internode 6, internode 16, leaf +3, calli and suspesion cells from sugarcane cultivar Q117 Each vegetative tissue was sampled from three independent field grown plants, pooled and sequenced using the GAIIx platform. Calli and suspension cell cultures were sequenced using the HiSeq 2000 platform.","dates":{"release":"2013-12-13T00:00:00Z","modification":"2023-09-04T05:38:00.789Z","creation":"2022-02-03T08:45:02.918Z"},"accession":"E-GEOD-53304","cross_references":{"ENA":["SRP034033"],"EFO":["EFO_0003737"]}}