biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsGlyn Bradleytranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-60880Experiment conducted to reverse engineer the transriptional response network of cytokines, growth factors and small molecular inhibitors in HLF cells. Global gene expression was analysed by microarrays HLF cells were incubated with each agent for 0.5 hours, 1 hour, 2 hours or 8 hours in standard conditions before total RNA was prepared and hybridised to Affymetrix microarraysbiostudies-arrayexpressScaning - GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.Sample Treatment - The agents were added to the cell culture medium and incubated for the desired time.Labeling - Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNANucleic Acid Extraction - Trizol extraction of total RNA according to the manufactures instructionsGrowth Protocol - Standard culturing conditionsHybridization - Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetwix U133A arrays.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsGlyn BradleyData Transformation - Data were analysed in R/Bioconductor, normalised by RMA ID_REF = VALUE = RMA valuefalseHuman Lung Fibroblasts treated with TGFbeta, IL1, EGF and small molecule inhibitors of TGFBR1 and p38Experiment conducted to reverse engineer the transriptional response network of cytokines, growth factors and small molecular inhibitors in HLF cells. Global gene expression was analysed by microarrays HLF cells were incubated with each agent for 0.5 hours, 1 hour, 2 hours or 8 hours in standard conditions before total RNA was prepared and hybridised to Affymetrix microarrays2014-08-29T00:00:00Z2023-09-10T19:42:15.095Z2022-02-03T14:14:22.068ZE-GEOD-60880GSE60880EFO_0002768