{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Tokuzo Arao"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-6379"],"description":["The 4　Fra2-targeting siRNA and 4 control-siRNA samples were used for analysis. Experiment Overall Design: A microarray procedure was performed according to the Affymetrix protocols (Santa Clara, CA). In brief, total RNA extracted from the tumor samples was analyzed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany) and cRNA was synthesized with the GeneChip® 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). The labeled cRNAs were then purified and used for probes. Hybridization was performed on the Affymetrix GeneChip HG-U133 Plus2.0 array for 16 hours at 45 ºC. Signal intensity was measured by a GeneChip®Scanner3000 (Affymetrix) and converted to numerical data with GeneChip Operating Software, Ver.1 (Affymetrix)."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Labeling - affymetrix protocol","Nucleic Acid Extraction - Affymetrix protocol","Labeling - Affymetrix protocol","Nucleic Acid Extraction - affymetrix protocol"],"figure_sub":["MIAME Score","Organization","Assays and Data","MAGE-TAB Files","Array Designs"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"pubmed_abstract":["Adult T-cell leukemia (ATL) is a mature CD4+ T-cell malignancy etiologically associated with human T-cell leukemia virus type 1 (HTLV-1). Primary ATL cells frequently express CCR4 at high levels. Since HTLV-1 Tax does not induce CCR4 expression, transcription factor(s) constitutively active in ATL may be responsible for its strong expression. We identified an activator protein-1 (AP-1) site in the CCR4 promoter as the major positive regulatory element in ATL cells. Among the AP-1 family members, Fra-2, JunB and JunD are highly expressed in fresh primary ATL cells. Consistently, the Fra-2/JunB and Fra-2/JunD heterodimers strongly activated the CCR4 promoter in Jurkat cells. Furthermore, Fra-2 small interfering RNA (siRNA) or JunD siRNA, but not JunB siRNA, effectively reduced CCR4 expressio"],"study_type":["RNAi profiling by array"],"species":["Homo sapiens"],"pubmed_title":["Aberrant expression of Fra-2 promotes CCR4 expression and cell proliferation in adult T-cell leukemia."],"pubmed_authors":["Hieshima Nakayama, Jin Arao, Shirakawa Nagakubo, Fujii Yamada, Kawada Oiso, Yoshie Nishio","Tokuzo Arao"],"additional_accession":[]},"is_claimable":false,"name":"siRNA profiling human adult T cell leukemia reveals Fra-2 promotes CCR4 expression and cell proliferation","description":"The 4　Fra2-targeting siRNA and 4 control-siRNA samples were used for analysis. Experiment Overall Design: A microarray procedure was performed according to the Affymetrix protocols (Santa Clara, CA). In brief, total RNA extracted from the tumor samples was analyzed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany) and cRNA was synthesized with the GeneChip® 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). The labeled cRNAs were then purified and used for probes. Hybridization was performed on the Affymetrix GeneChip HG-U133 Plus2.0 array for 16 hours at 45 ºC. Signal intensity was measured by a GeneChip®Scanner3000 (Affymetrix) and converted to numerical data with GeneChip Operating Software, Ver.1 (Affymetrix).","dates":{"release":"2008-06-14T00:00:00Z","modification":"2023-09-16T15:11:22.733Z","creation":"2022-03-10T06:11:55.273Z"},"accession":"E-GEOD-6379","cross_references":{"GEO":["GSE6379"],"pubmed":["18071306"],"EFO":["EFO_0001030"],"doi":["18071306"]}}