biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsSebastian Oedertranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-63962Human BEAS-2B bronchial epithelial cells were exposed directly at the air-liquid interphase towards exhaust gas and particles of a ship engine. The goal was to compare the responses towards different fuel combustions. The engine run either on diesel fuel (DF) or on Heavy Fuel Oil (HFO). The lung cells were exposed 3 times to each combustion aerosol (DF or HFO). The duration of the exposure was 4h. The cells were seeded into transwell-inserts 24h before exposure. Within each exposure 3 transwell-inserts were exposed to the complete aerosol and 3 transwell-inserts were exposed to the filtered aerosol. Effects of the complete aerosol were referenced against the filtered aerosol to determine the effects of the aerosol particles.biostudies-arrayexpressSample Treatment - In a fully automatic Air-liquid-interface exposure chamber cell cultures grown on porous membrane inserts, which supply the nutrient medium, are exposed directly to diluted aerosol from combustion sources (Fig. S2). The aerosol is humidified by steam injection to 85 % relative humidity and temperature is controlled to 37M-0C. The flow rate is 100 ml/min.Scaning - Scanning was performed according to the manufacturers protocol (Agilent, Waldbronn, Germany).Nucleic Acid Extraction - Total RNA was extracted using the AllPrep RNA/Protein Kit (Qiagen, Hilden, Germany).Growth Protocol - Human immortalized bronchial epithelial cells BEAS-2B (ATCCM-. CRL-9609M-bM-^DM-"; http://www.lgcstandards-atcc.org/Products/All/CRL-9609.aspx) were cultured in Bronchial Epithelial Growth Medium (BEGM, Lonza Inc., Walkersville, MD) supplemented with 100 units/ml penicillin/streptomycin and on plates that were pre-coated with 0.01 mg/ml fibronectin, 0.03 mg/ml bovine collagen Type 1 and 0.01 mg/ml BSA.Hybridization - Hybridisation to Sure Print G3 Human Gene Expression Microarrays (8x60k) was erformed according to the manufacturers protocol (Agilent, Waldbronn, Germany).Labeling - Total RNA was Cy3-labeled according to the manufacturers protocol (Agilent, Waldbronn, Germany).MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsSebastian OederData Transformation - The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters. ID_REF = VALUE = Normalized signal intensity.falseLung cell responses to ship diesel exhaust particlesHuman BEAS-2B bronchial epithelial cells were exposed directly at the air-liquid interphase towards exhaust gas and particles of a ship engine. The goal was to compare the responses towards different fuel combustions. The engine run either on diesel fuel (DF) or on Heavy Fuel Oil (HFO). The lung cells were exposed 3 times to each combustion aerosol (DF or HFO). The duration of the exposure was 4h. The cells were seeded into transwell-inserts 24h before exposure. Within each exposure 3 transwell-inserts were exposed to the complete aerosol and 3 transwell-inserts were exposed to the filtered aerosol. Effects of the complete aerosol were referenced against the filtered aerosol to determine the effects of the aerosol particles.2015-02-01T00:00:00Z2023-09-16T15:26:37.346Z2022-03-15T02:09:15.41ZE-GEOD-63962GSE63962EFO_0002768