biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsSvetlana Ghimbovschitranscription profiling by arrayMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-66739Currently there is growing concern with respect to scenarios where people are likely to be presented with radiation exposure along with many kinds of other injuries such as trauma and infection. The potential for such scenarios was brought to reality with the events and aftermath of the Fukushima nuclear disaster in Japan. As such medical complications arising from such exposures would be poorly dealt with as no evidence-based guidelines exist for their rehabilitation or recovery. Our research intends to differentially characterize combined radiation and burn injuries and identify novel pathways and biomarkers. Such findings will lead to better medical practices in the diagnosis, care and rehabilitation of affected individuals. The study includes four groups of mice: 1) Control sham mice group (n=4), 2) Skin burn injury mice group (n=6), 3) Radiation injury mice group (n=6), 4) Combined radiation and burn injury mice group (n=6). We propose to characterize the effects of combined radiation and burn injuries using microRNA microarray analysis. Our primary aim is to identify novel molecular pathways and biomarkers specific to whole blood samples (serum) from mice exposed to combined radiation and burn injuries. B6D2F1/J female mice will be used. 30 days following combined radiation and burn injuries arterial blood will be harvested from euthanized mice. 200ul of serum from whole blood samples will be used for microRNA microarray experiments (Affymetrix).biostudies-arrayexpressGrowth Protocol - B6D2F1/J female mice will be obtained from The Jackson Laboratory, Bar Harbor, Maine.Sample Treatment - All radiation and burn injuries will be performed at AFRRI Radiation and Animal facility as per IACUC approved protocols. 30 days following combined radiation and burn injuries arterial blood will be harvested from euthanized mice for genomic microRNA analysis.Scaning - The arrays were scanned with a Hewlett Packard G2500A gene Array Scanner.Hybridization - 21.5ul of each high-quality biotin-labeled miRNA sample was hybridized to Affymetrix Gene-Chip® miRNA 2.0 Array for 16 hours according to Affymetrix protocol. The arrays were washed and stained on the Affymetrix Fluidics station 400.Nucleic Acid Extraction - Mouse 200ul serum samples were subjected to total RNA isolation procedure using SeraMir Exosome RNA Amplification kit (System Biosciences, Mountain View, CA) according to manufacturer’s instructions. Concentration of each RNA sample was determined by NanoDrop® spectrophotometer ND-1000 (NanoDrop Technologies, Wilmington, DE). The quality of RNA samples was assessed with Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Santa Clara, CA).Labeling - Exosomal RNA samples, containing low molecular weight RNA, were biotin-labeled with FlashTag™ Biotin HSR RNA Labeling Kit. The quality of the biotin labeling process was confirmed by using Enzyme Linked Oligosorbent Assay (ELOSA) protocol (Affymetrix, Santa Clara, CA).MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsAminul IslamSvetlana GhimbovschiData Transformation - Gene expression values were obtained using Affymetrix Expression Console software, checked on Quality Control, normalized and analyzed using RMA-DABG option (Affymetrix, Santa Clara, CA). ID_REF = VALUE = Quantification DETECTION P-VALUE =falseIdentifying Non-Invasive Molecular Correlates relevant to Irradiation-Skin Burn Combined InjuriesCurrently there is growing concern with respect to scenarios where people are likely to be presented with radiation exposure along with many kinds of other injuries such as trauma and infection. The potential for such scenarios was brought to reality with the events and aftermath of the Fukushima nuclear disaster in Japan. As such medical complications arising from such exposures would be poorly dealt with as no evidence-based guidelines exist for their rehabilitation or recovery. Our research intends to differentially characterize combined radiation and burn injuries and identify novel pathways and biomarkers. Such findings will lead to better medical practices in the diagnosis, care and rehabilitation of affected individuals. The study includes four groups of mice: 1) Control sham mice group (n=4), 2) Skin burn injury mice group (n=6), 3) Radiation injury mice group (n=6), 4) Combined radiation and burn injury mice group (n=6). We propose to characterize the effects of combined radiation and burn injuries using microRNA microarray analysis. Our primary aim is to identify novel molecular pathways and biomarkers specific to whole blood samples (serum) from mice exposed to combined radiation and burn injuries. B6D2F1/J female mice will be used. 30 days following combined radiation and burn injuries arterial blood will be harvested from euthanized mice. 200ul of serum from whole blood samples will be used for microRNA microarray experiments (Affymetrix).2015-05-15T00:00:00Z2023-09-16T21:59:40.537Z2022-03-04T17:37:10.357ZE-GEOD-66739GSE66739EFO_0002768