{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Valerie LeBleu"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-66981"],"description":["Genetically engineered mice developed spontaneous pancreas cancer (Pdx-Cre;LSL-KRASG12D;P53Mut). Mice were also engineered to develop similar spontaneous pancreas cancer without Twist or Snail (conditional gene knockout). The pancreas tumors were harvested and analysed for gene expression profiles comparisons. Total RNA was isolated from the pancreas tumors of 2 mice with wild-type Twist and Snail, from 2 mice without Twist expression in the pancreas, and from 2 mice without Snail expression in the pancreas."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Scaning - Standard Illumina scanning protocol","Sample Treatment - Mice spontaneously developed pancreas tumors","Labeling - Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays","Hybridization - Standard Illumina hybridization protocol","Nucleic Acid Extraction - RNA was extracted with Trizol reagent,Quality control was performed with Agilent Bioanalyser."],"figure_sub":["MIAME Score","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"data_protocol":["Data Transformation - Genome Studio 5.0 ID_REF =  VALUE = background subtracted, rank invariant normalization Detection Pval ="],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"pubmed_abstract":["Diagnosis of pancreatic ductal adenocarcinoma (PDAC) is associated with a dismal prognosis despite current best therapies; therefore new treatment strategies are urgently required. Numerous studies have suggested that epithelial-to-mesenchymal transition (EMT) contributes to early-stage dissemination of cancer cells and is pivotal for invasion and metastasis of PDAC. EMT is associated with phenotypic conversion of epithelial cells into mesenchymal-like cells in cell culture conditions, although such defined mesenchymal conversion (with spindle-shaped morphology) of epithelial cells in vivo is rare, with quasi-mesenchymal phenotypes occasionally observed in the tumour (partial EMT). Most studies exploring the functional role of EMT in tumours have depended on cell-culture-induced loss-of-function and gain-of-function experiments involving EMT-inducing transcription factors such as Twist, Snail and Zeb1 (refs 2, 3, 7-10). Therefore, the functional contribution of EMT to invasion and metastasis remains unclear, and genetically engineered mouse models to address a causal connection are lacking. Here we functionally probe the role of EMT in PDAC by generating mouse models of PDAC with deletion of Snail or Twist, two key transcription factors responsible for EMT. EMT suppression in the primary tumour does not alter the emergence of invasive PDAC, systemic dissemination or metastasis. Suppression of EMT leads to an increase in cancer cell proliferation with enhanced expression of nucleoside transporters in tumours, contributing to enhanced sensitivity to gemcitabine treatment and increased overall survival of mice. Collectively, our study suggests that Snail- or Twist-induced EMT is not rate-limiting for invasion and metastasis, but highlights the importance of combining EMT inhibition with chemotherapy for the treatment of pancreatic cancer."],"study_type":["transcription profiling by array"],"species":["Mus musculus"],"pubmed_title":["Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer."],"pubmed_authors":["Zheng X, Carstens JL, Kim J, Scheible M, Kaye J, Sugimoto H, Wu CC, LeBleu VS, Kalluri R","Valerie LeBleu"],"additional_accession":[]},"is_claimable":false,"name":"Genome-wide analysis of gene expression in mouse pancreas tumors","description":"Genetically engineered mice developed spontaneous pancreas cancer (Pdx-Cre;LSL-KRASG12D;P53Mut). Mice were also engineered to develop similar spontaneous pancreas cancer without Twist or Snail (conditional gene knockout). The pancreas tumors were harvested and analysed for gene expression profiles comparisons. Total RNA was isolated from the pancreas tumors of 2 mice with wild-type Twist and Snail, from 2 mice without Twist expression in the pancreas, and from 2 mice without Snail expression in the pancreas.","dates":{"release":"2015-11-20T00:00:00Z","modification":"2023-09-12T09:59:26.379Z","creation":"2021-10-04T17:28:55Z"},"accession":"E-GEOD-66981","cross_references":{"GEO":["GSE66981"],"pubmed":["26560028"],"EFO":["EFO_0002768"],"doi":["10.1038/nature16064"]}}