biostudies-arrayexpressEzequiel CalvoHomo sapiensMicroArraySuite 5.0https://www.ebi.ac.uk/biostudies/studies/E-GEOD-7223Androgen-induced bZIP (AIbZIP) is a basic leucine zipper (bZIP); transcription factor that is encoded by the CREB3L4 (cAMP responsive element; binding protein 3-like 4) gene. In humans, AIbZIP mRNA is most abundant in; the prostate but it is also present, albeit at much lower levels, in the; pancreas and other tissues. The full-length AIbZIP protein localizes to the; endoplasmic reticulum and the available data suggest that AIbZIP is; processed to its transcriptionally active form by regulated intramembrane; proteolysis (RIP). To define the transcriptional programme that is initiated; by AIbZIP, we generated LNCaP prostate cancer cell lines that conditionally; express the processed form of AIbZIP. The recombinant protein consists of; the first 290 amino acids of AIbZIP fused to a C-terminabiostudies-arrayexpressHybridization - Title: Affymetrix Generic Hybridization. Description:Nucleic Acid Extraction - Total RNA was isolated from cells using Trizol Reagent, according to the manufacturer’s protocol (Invitrogen).Growth Protocol - RPMI 1640 supplemented with 10% fetal calf serum, 2 mM L-glutamine, 50 ug streptomycin/ml and 100 IU penicillin/ml. Cells were grown at 37 C in 5% CO2.Sample Processing - Cells were incubated in medium containing vehicle alone (Control) or in medium containing RSL1 (500 nM) for 6 or 24 hr.Labeling - Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).MIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsFeature Extraction - Title: Affymetrix CEL analysis. Description:MetabolomicsUnknownTranscriptomicsGenomicsProteomicsThe androgen-regulated protein androgen-induced bZIP (AIbZIP) is a bZIP transcription factor that localizes to the membrane of the endoplasmic reticulum (ER). The physiological role of AIbZIP is unknown, but other ER-bound transcription factors such as ATF6 and SREBPs play a crucial role in the regulation of protein processing and lipid synthesis, respectively. In response to alterations in the intracellular milieu, ATF6 and SREBPs are processed to their transcriptionally active forms by regulated intramembrane proteolysis. In humans, AIbZIP mRNA is expressed in several organs including the pancreas, liver, and gonads, but it is especially abundant in prostate epithelial cells. We therefore used LNCaP human prostate cancer cells as a model to identify stimuli that lead to AIbZIP activationtranscription profiling by arrayHomo sapiensTranscriptional profiling of genes that are regulated by the endoplasmic reticulum-bound transcription factor AIbZIP/CREB3L4 in prostate cells.Sonia Ben Aicha, Julie Lessard, Mélissa Pelletier, Andréa Fournier, Ezequiel Calvo, Claude LabrieEzequiel CalvofalseTranscription profiling of human LNCaP cell lines transfected with AIbZIPAndrogen-induced bZIP (AIbZIP) is a basic leucine zipper (bZIP); transcription factor that is encoded by the CREB3L4 (cAMP responsive element; binding protein 3-like 4) gene. In humans, AIbZIP mRNA is most abundant in; the prostate but it is also present, albeit at much lower levels, in the; pancreas and other tissues. The full-length AIbZIP protein localizes to the; endoplasmic reticulum and the available data suggest that AIbZIP is; processed to its transcriptionally active form by regulated intramembrane; proteolysis (RIP). To define the transcriptional programme that is initiated; by AIbZIP, we generated LNCaP prostate cancer cell lines that conditionally; express the processed form of AIbZIP. The recombinant protein consists of; the first 290 amino acids of AIbZIP fused to a C-termina2008-06-15T00:00:00Z2023-09-14T00:22:40.468Z2022-03-04T15:24:59.626ZE-GEOD-7223GSE722317712038EFO_000276817712038