biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsClaude Lachancetranscription profiling by arraySus scrofaSus scrofahttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-75687We investigated for the first time the in vitro response of PRRSV-infected porcine DCs and monocytes to S. suis. We first assessed the effect of PRRSV infection on the phagocytosis and intracellular survival of S. suis by these two cell populations. We then used a genomic approach to compare the gene expression profiles of both type of cells infected with S. suis, with or without a previous infection with PRRSV. Total RNA obtained from porcine monocytes and dendritic cells infected with S. suis, PRRSV, or S. suis & PRRSV. Four replicates in all groups.biostudies-arrayexpressHybridization - Standard Agilent hybridization protocolScaning - Standard Agilent scanning protocolSample Treatment - Cells were plated at 1 x 10^6 cells/ml in 1 ml of complete RPMI and rested 3 h before use. For PRRSV infection, medium was removed and replaced with 300 μl of serum-free complete RPMI with PRRSV IAF-Klop (MOI of 0.1) for 2 h. The wells were washed with PBS twice to remove free virus and 1 ml of complete RPMI was added to each well.For co-infection experiments, cells were infected with PRRSV as described above. After 2 h infection, cells were washed with PBS. Then, S. suis P1/7 was added at a MOI of 0.1 (1 x 105 CFU) for the microarray assay. Bacteria were left in culture with the cells for 12 h. In parallel studies, virus-infected cells were left without bacterial infection (virus infection alone) or were virus-mock infected, similarly incubated and then infected with S. suis (bacterial infection alone).Nucleic Acid Extraction - Total RNA was extracted from porcine monocytes using QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.Labeling - cDNA was labelled with Cyanine 3 Labeling Master Mix according to Manufacturer's protocol.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsGaël AurayClaude LachanceCarl GagnonMarcelo GottschalkMariela SeguraYingchao WangData Transformation - The data were normalised with VSN method using Flexarray 1.6.2 ID_REF = VALUE = VSN normalized signal intensityfalseTranscriptional immune response of PRRSV-infected porcine dendritic cells and monocytes to Streptococcus suis infectionWe investigated for the first time the in vitro response of PRRSV-infected porcine DCs and monocytes to S. suis. We first assessed the effect of PRRSV infection on the phagocytosis and intracellular survival of S. suis by these two cell populations. We then used a genomic approach to compare the gene expression profiles of both type of cells infected with S. suis, with or without a previous infection with PRRSV. Total RNA obtained from porcine monocytes and dendritic cells infected with S. suis, PRRSV, or S. suis & PRRSV. Four replicates in all groups.2016-06-01T00:00:00Z2023-09-17T22:02:54.718Z2021-10-05T19:28:49ZE-GEOD-75687GSE75687EFO_0002768