{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["William Evans"],"study_type":["transcription profiling by array"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-GEOD-76849"],"description":["In primary leukemia cells there is a significant over-representation of positively correlated microRNA and mRNA expression (see GSE66708) for genes containing sequences favoring microRNA-duplex DNA triplex formation, suggesting this as a mechanism by which microRNA may enhance gene transcription. RNA was extracted from fresh leukemic blasts and hybridized to a panel of miRNA probes."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Hybridization - hybridization was done at 56 degrees Celsius for 16 hours followed by washing on an HS Pro instrument from Tecan.","Nucleic Acid Extraction - Trizol extraction of total RNA was performed according to the manufacturer's instructions.","Scaning - Scanning was performed using an Agilent G2505B scanning system","Labeling - miRCURY Power labeling kit used according to manufacturer's (Exiqon) instructions"],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","MAGE-TAB Files","Array Designs"],"pubmed_authors":["Evans William","William Evans","Paugh Steve","Bonten Erik"],"data_protocol":["Data Transformation - raw counts were processed using the limma package(version 3.6.9) from Bioconductor ID_REF =  VALUE = processed signal intensity"],"additional_accession":[]},"is_claimable":false,"name":"miRNA from leukemia cells","description":"In primary leukemia cells there is a significant over-representation of positively correlated microRNA and mRNA expression (see GSE66708) for genes containing sequences favoring microRNA-duplex DNA triplex formation, suggesting this as a mechanism by which microRNA may enhance gene transcription. RNA was extracted from fresh leukemic blasts and hybridized to a panel of miRNA probes.","dates":{"release":"2016-01-20T00:00:00Z","modification":"2023-10-03T14:29:20.553Z","creation":"2022-01-28T22:49:43.058Z"},"accession":"E-GEOD-76849","cross_references":{"GEO":["GSE76849"],"EFO":["EFO_0002768"]}}