biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsWilliam Evanstranscription profiling by arrayHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-GEOD-76849In primary leukemia cells there is a significant over-representation of positively correlated microRNA and mRNA expression (see GSE66708) for genes containing sequences favoring microRNA-duplex DNA triplex formation, suggesting this as a mechanism by which microRNA may enhance gene transcription. RNA was extracted from fresh leukemic blasts and hybridized to a panel of miRNA probes.biostudies-arrayexpressHybridization - hybridization was done at 56 degrees Celsius for 16 hours followed by washing on an HS Pro instrument from Tecan.Nucleic Acid Extraction - Trizol extraction of total RNA was performed according to the manufacturer's instructions.Scaning - Scanning was performed using an Agilent G2505B scanning systemLabeling - miRCURY Power labeling kit used according to manufacturer's (Exiqon) instructionsMIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsEvans WilliamWilliam EvansPaugh SteveBonten ErikData Transformation - raw counts were processed using the limma package(version 3.6.9) from Bioconductor ID_REF = VALUE = processed signal intensityfalsemiRNA from leukemia cellsIn primary leukemia cells there is a significant over-representation of positively correlated microRNA and mRNA expression (see GSE66708) for genes containing sequences favoring microRNA-duplex DNA triplex formation, suggesting this as a mechanism by which microRNA may enhance gene transcription. RNA was extracted from fresh leukemic blasts and hybridized to a panel of miRNA probes.2016-01-20T00:00:00Z2023-10-03T14:29:20.553Z2022-01-28T22:49:43.058ZE-GEOD-76849GSE76849EFO_0002768