biostudies-arrayexpressJan LohmannArabidopsis thalianaMicroArraySuite 5.0https://www.ebi.ac.uk/biostudies/studies/E-MEXP-1100Comparison of Arabidopsis seedlings with disturbed function of CDKB2;1 and CDKB2;2 by either overexpression or knock-downbiostudies-arrayexpressNucleic Acid Extraction - Total RNA extraction using Qiagen RNeasy Plant Mini KitHybridization - Title: Affymetrix Generic Hybridization. Description:Labeling - Title: Affymetrix in vitro transcription. Description:Growth Protocol - plants were grown on soil at 20° C in continuous lightMIAME ScoreRaw DataOrganizationAssays and DataProcessed DataAdditional FilesMAGE-TAB FilesArray DesignsAssay Data Transformation - Title: Affymetrix CHP Analysis (ExpressionStat). Description:Feature Extraction - Title: Affymetrix CEL analysis. Description:MetabolomicsUnknownTranscriptomicsGenomicsProteomicsTo maintain proper meristem function, cell division and differentiation must be coordinately regulated in distinct subdomains of the meristem. Although a number of regulators necessary for the correct organization of the shoot apical meristem (SAM) have been identified, it is still largely unknown how their function is integrated with the cell cycle machinery to translate domain identity into correct cellular behavior. We show here that the cyclin-dependent kinases CDKB2;1 and CDKB2;2 are required both for normal cell cycle progression and for meristem organization. Consistently, the CDKB2 genes are highly expressed in the SAM in a cell cycle-dependent fashion, and disruption of CDKB2 function leads to severe meristematic defects. In addition, strong alterations in hormone signaling both atranscription profiling by arrayArabidopsis thalianaRequirement of B2-Type Cyclin-Dependent Kinases for Meristem Integrity in Arabidopsis thalianaAndersen, Stig Uggerhoj; Buechel, Sabine; Zhao, Zhong; Ljung, Karin; Novak, Ondrej; Busch, Wolfgang; Schuster, Christoph; Lohmann, Jan U.Jan LohmannfalseTranscription profiling of Arabidopsis seedlings with disturbed function of CDKB2;1 and CDKB2;2 by either overexpression or knock-downComparison of Arabidopsis seedlings with disturbed function of CDKB2;1 and CDKB2;2 by either overexpression or knock-down2007-11-01T00:00:00Z2022-11-21T15:30:22.92Z2022-02-03T12:19:30.866ZE-MEXP-110018223038EFO_000276818223038