biostudies-arrayexpressThilo BorchardtMus musculusMicroArraySuite 5.0https://www.ebi.ac.uk/biostudies/studies/E-MEXP-1486Expression profiling of E14,5 stage Embryos of two different genotypes.biostudies-arrayexpressNucleic Acid Extraction - TRIzolGrowth Protocol - Mouse embryonic stage 14,5Labeling - Title: Affymetrix in vitro transcription. Description:Sample Processing - Homogenization and Isolation of total RNA wirth TRIZOL according to manufaturers manualMIAME ScoreRaw DataOrganizationAssays and DataProcessed DataAdditional FilesMAGE-TAB FilesArray DesignsAssay Data Transformation - Title: Affymetrix CHP Analysis (ExpressionStat). Description:Feature Extraction - Title: Affymetrix CEL analysis. Description:MetabolomicsUnknownTranscriptomicsGenomicsProteomicsThe development of myogenic cells is mainly determined by expression of two myogenic factors, Myf5 and Myod1 (MyoD), which genetically compensate for each other during embryogenesis. Here, we demonstrate by conditional cell ablation in mice that Myf5 determines a distinct myogenic cell population, which also contains some Myod1-positive cells. Ablation of this lineage uncovers the presence of a second autonomous myogenic lineage, which superseded Myf5-dependent myogenic cells and expressed Myod1. By contrast, ablation of myogenin-expressing cells erased virtually all differentiated muscle cells, indicating that some aspects of the myogenic program are shared by most skeletal muscle cells. We conclude that Myf5 and Myod1 define different cell lineages with distinct contributions to muscle precursor cells and differentiated myotubes. Individual myogenic cell lineages seem to substitute for each other within the developing embryo.transcription profiling by arrayMus musculusDifferent autonomous myogenic cell populations revealed by ablation of Myf5-expressing cells during mouse embryogenesisGensch, Nicole; Borchardt, Thilo; Schneider, Andre; Riethmacher, Dieter; Braun, ThomasThilo BorchardtfalseTranscription profiling of mouse E14.5 stage embryos with Myf5-Cre x DT-A ROSA knock in or Nyf5-Cre genotypesExpression profiling of E14,5 stage Embryos of two different genotypes.2008-03-06T00:00:00Z2022-11-21T06:46:07.64Z2022-03-09T07:07:42.576ZE-MEXP-148618367555EFO_000276818367555