{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Claus Nerlov"],"organism":["Mus musculus"],"software":["MicroArraySuite 5.0"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MEXP-1719"],"description":["Effect of CEBPalpha and CEBPbeta null and/or mutant alleles on epidermal development"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Hybridization - Title: Affymetrix Generic Hybridization. Description:","Labeling - Title: Affymetrix in vitro transcription. Description:","Nucleic Acid Extraction - Total RNA was extracted using TRIzol reagent (Invitrogen) and further purified with RNeasy Mini Kits (Qiagen).  <br>(Parameters: Extracted product = total_RNA, Amplification = RNA polymerases)"],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","Processed Data","Additional Files","MAGE-TAB Files","Array Designs"],"data_protocol":["Feature Extraction - Title: Affymetrix CEL analysis. Description:"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"pubmed_abstract":["The transcriptional regulators that couple interfollicular basal keratinocyte proliferation arrest to commitment and differentiation are yet to be identified. Here we report that the basic region leucine zipper transcription factors C/EBPalpha and C/EBPbeta are co-expressed in basal keratinocytes, and are coordinately upregulated as keratinocytes exit the basal layer and undergo terminal differentiation. Mice lacking both C/EBPalpha and beta in the epidermis showed increased proliferation of basal keratinocytes and impaired commitment to differentiation. This led to ectopic expression of keratin 14 (K14) and DeltaNp63 in suprabasal cells, decreased expression of spinous and granular layer proteins, parakeratosis and defective epidermal water barrier function. Knock-in mutagenesis revealed "],"study_type":["transcription profiling by array"],"species":["Mus musculus"],"pubmed_title":["C/EBPalpha and beta couple interfollicular keratinocyte proliferation arrest to commitment and terminal differentiation."],"pubmed_authors":["Claus Nerlov","Lopez RG, Garcia-Silva S, Moore SJ, Bereshchenko O, Martinez-Cruz AB, Ermakova O, Kurz E, Paramio JM, Nerlov C."],"additional_accession":[]},"is_claimable":false,"name":"Transcription profiling of mouse CEBPalpha and CEBPbeta null and/or mutant alleles to investigate epidermal development","description":"Effect of CEBPalpha and CEBPbeta null and/or mutant alleles on epidermal development","dates":{"release":"2009-09-21T00:00:00Z","modification":"2022-11-22T06:49:30.041Z","creation":"2021-10-01T18:13:07Z"},"accession":"E-MEXP-1719","cross_references":{"pubmed":["19749746"],"EFO":["EFO_0002768"],"doi":["19749746"]}}