{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Chang-Gong Liu"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MEXP-224"],"description":["microRNA expression reproducibility studies on chip"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Labeling - The 5 prime biotin labeled oligo primers were used for first strand cDNA synthesis.","Hybridization - The chips were hybridized in special condition for the short labeled targets","Nucleic Acid Extraction - The RNA were extracted using TriZol standard protocol"],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","MAGE-TAB Files","Array Designs"],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"pubmed_abstract":["MicroRNAs (miRNAs) are a class of small noncoding RNA genes recently found to be abnormally expressed in several types of cancer. Here, we describe a recently developed methodology for miRNA gene expression profiling based on the development of a microchip containing oligonucleotides corresponding to 245 miRNAs from human and mouse genomes. We used these microarrays to obtain highly reproducible results that revealed tissue-specific miRNA expression signatures, data that were confirmed by assessment of expression by Northern blots, real-time RT-PCR, and literature search. The microchip oligolibrary can be expanded to include an increasing number of miRNAs discovered in various species and is useful for the analysis of normal and disease states."],"study_type":["microRNA profiling by array"],"species":["Mus musculus"],"pubmed_title":["An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues"],"pubmed_authors":["Carlo Croce","Brian Meloon","Liu, Chang-Gong; Calin, George Adrian; Meloon, Brian; Gamliel, Nir; Sevignani, Cinzia; Ferracin, Manuela; Dumitru, Calin Dan; Shimizu, Masayoshi; Zupo, Simona; Dono, Mariella; Alder, Hansjuerg; Bullrich, Florencia; Negrini, Massimo; Croce, Carlo M.","Chang-Gong Liu","Cinzia Sevignani","George Calin","changgong Liu","Nir Gamliel"],"additional_accession":[]},"is_claimable":false,"name":"MicroRNA profiling of a range of human cell types and tissues to determine reproducibility","description":"microRNA expression reproducibility studies on chip","dates":{"release":"2004-12-31T00:00:00Z","modification":"2021-10-01T18:39:24Z","creation":"2021-10-01T18:39:24Z"},"accession":"E-MEXP-224","cross_references":{"pubmed":["15210942"],"EFO":["EFO_0000753"],"doi":["15210942"]}}