{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Carlo Croce"],"disease":["chronic lymphocytic leukemia"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MEXP-227"],"description":["The microRNA expression patterns in 41 B-CLL samples were identified by comparison with normal CD5 positive cells"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Labeling - The 5 prime biotin labeled oligo primers were used for first strand cDNA synthesis.","Hybridization - The chips were hybridized in special condition for the short labeled targets","Nucleic Acid Extraction - The RNA were extracted using TriZol standard protocol"],"figure_sub":["MIAME Score","Raw Data","Organization","Assays and Data","MAGE-TAB Files","Array Designs"],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"pubmed_abstract":["Little is known about the expression levels or function of micro-RNAs (miRNAs) in normal and neoplastic cells, although it is becoming clear that miRNAs play important roles in the regulation of gene expression during development [Ambros, V. (2003) Cell 113, 673-676; McManus, M. T. (2003) Semin. Cancer Biol. 13, 253-258]. We now report the genomewide expression profiling of miRNAs in human B cell chronic lymphocytic leukemia (CLL) by using a microarray containing hundreds of human precursor and mature miRNA oligonucleotide probes. This approach allowed us to identify significant differences in miRNome expression between CLL samples and normal CD5+ B cells; data were confirmed by Northern blot analyses and real-time RT-PCR. At least two distinct clusters of CLL samples can be identified tha"],"study_type":["microRNA profiling by array"],"species":["Homo sapiens"],"pubmed_title":["MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias"],"pubmed_authors":["Carlo Croce","George Calin; Chang-Gong Liu; Carlo Croce","Chang-Gong Liu","George Calin","changgong Liu"],"additional_accession":[]},"is_claimable":false,"name":"MicroRNA profiling of human 41 B-CLL samples vs. normal CD5 positive cells to identify microRNA expression patterns","description":"The microRNA expression patterns in 41 B-CLL samples were identified by comparison with normal CD5 positive cells","dates":{"release":"2004-12-31T00:00:00Z","modification":"2022-02-02T18:01:52.195Z","creation":"2022-02-02T18:01:52.195Z"},"accession":"E-MEXP-227","cross_references":{"pubmed":["15284443"],"EFO":["EFO_0000753"],"doi":["15284443"]}}