biostudies-arrayexpressCarlo Crocechronic lymphocytic leukemiaHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MEXP-227The microRNA expression patterns in 41 B-CLL samples were identified by comparison with normal CD5 positive cellsbiostudies-arrayexpressLabeling - The 5 prime biotin labeled oligo primers were used for first strand cDNA synthesis.Hybridization - The chips were hybridized in special condition for the short labeled targetsNucleic Acid Extraction - The RNA were extracted using TriZol standard protocolMIAME ScoreRaw DataOrganizationAssays and DataMAGE-TAB FilesArray DesignsMetabolomicsUnknownTranscriptomicsGenomicsProteomicsLittle is known about the expression levels or function of micro-RNAs (miRNAs) in normal and neoplastic cells, although it is becoming clear that miRNAs play important roles in the regulation of gene expression during development [Ambros, V. (2003) Cell 113, 673-676; McManus, M. T. (2003) Semin. Cancer Biol. 13, 253-258]. We now report the genomewide expression profiling of miRNAs in human B cell chronic lymphocytic leukemia (CLL) by using a microarray containing hundreds of human precursor and mature miRNA oligonucleotide probes. This approach allowed us to identify significant differences in miRNome expression between CLL samples and normal CD5+ B cells; data were confirmed by Northern blot analyses and real-time RT-PCR. At least two distinct clusters of CLL samples can be identified thamicroRNA profiling by arrayHomo sapiensMicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemiasCarlo CroceGeorge Calin; Chang-Gong Liu; Carlo CroceChang-Gong LiuGeorge Calinchanggong LiufalseMicroRNA profiling of human 41 B-CLL samples vs. normal CD5 positive cells to identify microRNA expression patternsThe microRNA expression patterns in 41 B-CLL samples were identified by comparison with normal CD5 positive cells2004-12-31T00:00:00Z2022-02-02T18:01:52.195Z2022-02-02T18:01:52.195ZE-MEXP-22715284443EFO_000075315284443